Recombinant
RabMAb

Recombinant Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)

Overview

  • Product name

    Anti-IL-17A antibody [EPR21776] - BSA and Azide free
    See all IL-17A primary antibodies
  • Description

    Rabbit monoclonal [EPR21776] to IL-17A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse IL-17A aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q62386

  • Positive control

    • WB: EL4 cells treated with PMA, ionomycin and Brefeldin A, whole cell lysate.
  • General notes

    Ab231891 is the carrier-free version of ab218013. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21776
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14,17 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • interleukin 17A antibody
    • Interleukin-17A antibody
    • interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Images

  • All lanes : Anti-IL-17A antibody [EPR21776] (ab218013) at 1/1000 dilution

    Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 2 : EL4 treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin (ab120116) for 6 hours, then with 500 ng/ml Brefeldin A for 18 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa
    Observed band size: 14,17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Expression of IL17A can be induced by PMA and Ionomycin treatment (PMID 28382171).

    The expression profile is consistent with the literature (PMID 9764847).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218013).

References

ab231891 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab231891.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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