Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21776] to IL-17A - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Product nameAnti-IL-17A antibody [EPR21776] - BSA and Azide free
See all IL-17A primary antibodies
DescriptionRabbit monoclonal [EPR21776] to IL-17A - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse IL-17A aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q62386
- WB: EL4 cells treated with PMA, ionomycin and Brefeldin A, whole cell lysate.
Ab231891 is the carrier-free version of ab218013. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab231891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab231891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 14,17 kDa (predicted molecular weight: 17 kDa).|
FunctionInduces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
Tissue specificityRestricted to activated memory T-cells.
Sequence similaritiesBelongs to the IL-17 family.
modificationsFound both in glycosylated and nonglycosylated forms.
- Information by UniProt
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
All lanes : Anti-IL-17A antibody [EPR21776] (ab218013) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin (ab120116) for 6 hours, then with 500 ng/ml Brefeldin A for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 14,17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/dilution buffer and concentration: 5% NFDM/TBST
Expression of IL17A can be induced by PMA and Ionomycin treatment (PMID 28382171).
The expression profile is consistent with the literature (PMID 9764847).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218013).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231891 has not yet been referenced specifically in any publications.