Recombinant
RabMAb

Recombinant Anti-IL-17F antibody [EPR17830-169] (ab187059)

Overview

  • Product name

    Anti-IL-17F antibody [EPR17830-169]
    See all IL-17F primary antibodies
  • Description

    Rabbit monoclonal [EPR17830-169] to IL-17F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse IL-17F aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q7TNI7

  • Positive control

    • WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cytometry: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
Flow Cyt 1/60.
WB 1/1000. Predicted molecular weight: 18 kDa.

Target

  • Function

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificity

    Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in disease

    Candidiasis, familial, 6
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CANDF6 antibody
    • Cytokine ML 1 antibody
    • Cytokine ML-1 antibody
    • IL 17F antibody
    • IL 24 antibody
    • IL-17F antibody
    • IL-24 antibody
    • Il17f antibody
    • IL17F_HUMAN antibody
    • Interleukin 17F antibody
    • Interleukin 24 antibody
    • Interleukin-17F antibody
    • Interleukin-24 antibody
    • ML 1 antibody
    • ML1 antibody
    • Mutant IL 17F antibody
    • OTTHUMP00000016602 antibody
    see all

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml Ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.

    Secondary antibody used Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

  • All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution

    Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
    Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST

  • IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
    Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds

  • All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution

    Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 16-18 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST

    The molecular mass observed is consistent with the literature (PMID 2212322).

    Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).

References

ab187059 has not yet been referenced specifically in any publications.

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