Recombinant Anti-IL-17F antibody [EPR17830-169] (ab187059)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17830-169] to IL-17F
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-17F antibody [EPR17830-169]
See all IL-17F primary antibodies -
Description
Rabbit monoclonal [EPR17830-169] to IL-17F -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cyt (intra): EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17830-169 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/60.
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IP |
1/30.
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WB |
1/1000. Predicted molecular weight: 18 kDa.
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Notes |
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Flow Cyt (Intra)
1/60. |
IP
1/30. |
WB
1/1000. Predicted molecular weight: 18 kDa. |
Target
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 257630 Mouse
- SwissProt: Q7TNI7 Mouse
- Unigene: 222807 Mouse
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Alternative names
- CANDF6 antibody
- Cytokine ML 1 antibody
- Cytokine ML-1 antibody
see all
Images
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500ng/ml Ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.
Secondary antibody used Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.
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All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution
Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST
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IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.
Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds
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All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 16-18 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking/Diluting buffer: 5% NFDM/TBST
The molecular mass observed is consistent with the literature (PMID 2212322).
Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab187059 has not yet been referenced specifically in any publications.