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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
  • Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-IL-17RA Receptor antibody [EPR22831-5]
    See all IL-17RA Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IL-17RA Receptor aa 1-350. The exact sequence is proprietary.
    Database link: Q96F46

  • Positive control

    • WB: Wild-type HAP1, THP-1 and K-562 lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22831-5
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Alternative Versions

    • Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Applications

Our Abpromise guarantee covers the use of ab263908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 96 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling.
    • Tissue specificity

      Widely expressed.
    • Involvement in disease

      Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
    • Sequence similarities

      Contains 1 SEFIR domain.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q96F46 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23765 Human
      • Omim: 605461 Human
      • SwissProt: Q96F46 Human
      • Unigene: 48353 Human
      • Alternative names

        • CANDF5 antibody
        • CD217 antibody
        • CD217 antigen antibody
        • CDw217 antibody
        • CTLA8 antibody
        • HIL 17R antibody
        • hIL17R antibody
        • I17RA_HUMAN antibody
        • IL 17 receptor A antibody
        • IL 17 receptor antibody
        • IL 17RA antibody
        • IL-17 receptor A antibody
        • IL-17RA antibody
        • IL17 antibody
        • IL17A receptor antibody
        • IL17R antibody
        • IL17RA antibody
        • IMD51 antibody
        • Interleukin 17 receptor A antibody
        • Interleukin-17 receptor A antibody
        • MGC10262 antibody
        see all

      Images

      • Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
        Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
        All lanes : Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908) at 1/1000 dilution

        Lane 1 : Wild-type HAP1 whole cell lysate
        Lane 2 : IL-17RA Receptor knockout HAP1 whole cell lysate
        Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
        Lane 4 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
        Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 96 kDa
        Observed band size: 120-170 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST

        ab263908 was shown to specifically react with IL-17RA Receptor in wild-type HAP1 cells as signal was lost in IL-17RA Receptor knockout cells. Wild-type and IL-17RA Receptor knockout samples were subjected to SDS-PAGE. ab263908 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

        The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30604628)

        Exposure time: 37 seconds

      • Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
        Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab263908? Please let us know so that we can cite the reference in this datasheet.

      ab263908 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab263908.
      Please use the links above to contact us or submit feedback about this product.

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      For licensing inquiries, please contact partnerships@abcam.com

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