Recombinant Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
Overview
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Product name
Anti-IL-17RA Receptor antibody [EPR22831-5]
See all IL-17RA Receptor primary antibodies -
Description
Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IL-17RA Receptor aa 1-350. The exact sequence is proprietary.
Database link: Q96F46 -
Positive control
- WB: Wild-type HAP1, THP-1 and K-562 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22831-5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab263908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 96 kDa. |
Target
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Function
Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 SEFIR domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 23765 Human
- Omim: 605461 Human
- SwissProt: Q96F46 Human
- Unigene: 48353 Human
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Alternative names
- CANDF5 antibody
- CD217 antibody
- CD217 antigen antibody
see all
Images
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![Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)](https://www.abcam.com/ps/products/263/ab263908/Images/ab263908-366154-anti-il-17ra-receptor-antibody-epr22831-5-western-blot-human.jpg)
Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)All lanes : Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IL-17RA Receptor knockout HAP1 whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 120-170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
ab263908 was shown to specifically react with IL-17RA Receptor in wild-type HAP1 cells as signal was lost in IL-17RA Receptor knockout cells. Wild-type and IL-17RA Receptor knockout samples were subjected to SDS-PAGE. ab263908 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30604628)
Exposure time: 37 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab263908 has not yet been referenced specifically in any publications.
