Recombinant
RabMAb

Recombinant Anti-IL-18 antibody [EPR19954-188] (ab243091)

Submit a review Submit a question

Overview

  • Product name

    Anti-IL-18 antibody [EPR19954-188]
    See all IL-18 primary antibodies

  • Description

    Rabbit monoclonal [EPR19954-188] to IL-18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14116

  • Positive control

    • WB: PC-3, HeLa and HaCaT whole cell lysates. IHC-P: Human tonsil, liver and kidney tissues. Flow cyt: PC-3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab243091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22, 14 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

    • Sequence similarities

      Belongs to the IL-1 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Iboctadekin antibody
        • IFN gamma inducing factor antibody
        • IFN-gamma-inducing factor antibody
        • IGIF antibody
        • IL 1 gamma antibody
        • IL 18 antibody
        • IL 1g antibody
        • IL-1 gamma antibody
        • IL-18 antibody
        • IL1 gamma antibody
        • IL18 antibody
        • IL18 protein antibody
        • IL18_HUMAN antibody
        • IL1F4 antibody
        • IL1g antibody
        • IL1gamma antibody
        • ILIF4 antibody
        • Interferon gamma inducing factor antibody
        • Interferon gamma-inducing factor antibody
        • Interleukin 1 gamma antibody
        • Interleukin 18 (interferon-gamma-inducing factor) antibody
        • Interleukin 18 antibody
        • Interleukin-1 gamma antibody
        • Interleukin-18 antibody
        • Interleukin18 antibody
        • MGC12320 antibody
        see all

      Images

      • Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        All lanes : Anti-IL-18 antibody [EPR19954-188] (ab243091) at 1/1000 dilution

        Lane 1 : Wild-type cell lysate
        Lane 2 : knockout cell lysate

        Performed under reducing conditions.

        Predicted band size: 22 kDa
        Observed band size: 22 kDa



        Lanes 1-2: Merged signal (red and green). Green - ab243091 observed at 22 kDa. Red - loading control ab8245 observed at 37 kDa.

        ab243091 Anti-IL-18 antibody [EPR19954-188] was shown to specifically react with IL-18 in HeLa wild type cells. Loss of signal was observed when knockout sample ab256952 was used. Wild type and IL-18 knockout samples were subjected to SDS-PAGE. ab243091 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)

        Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on distal convoluted tubules of human kidney (PMID: 17687255). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells in human liver (PMID: 19084941). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on subsets of macrophages in tonsil (PMID: 28842466). Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] (ab243091)

        Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and PC-3 (human prostate adenocarcinoma cell line) (right panel) cells labeling IL-18 with ab243091 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

        Negative control: Jurkat (PMID 15086390).

      • Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)
        All lanes : Anti-IL-18 antibody [EPR19954-188] (ab243091) at 1/1000 dilution

        Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
        Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
        Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 22 kDa
        Observed band size: 14,22 kDa
        why is the actual band size different from the predicted?



        Exposure time :  Lanes 1 & 2: 3 minutes. Lane 3: 26 seconds.

        Blocking and dilution buffer: 5% NFDM/TBST. 

        The bands observed are consistent with what has been described in the literature. Mature IL-18 protein is detected as a band at 18 kDa, whereas the 14-kDa band represents
        the inactive form (PMID: 15326478).

      References

      ab243091 has not yet been referenced specifically in any publications.

      Publishing research using ab243091? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab243091.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com