Recombinant Anti-IL-18 antibody [EPR19954-188] (ab243091)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-IL-18 antibody [EPR19954-188]
See all IL-18 primary antibodies -
DescriptionRabbit monoclonal [EPR19954-188] to IL-18
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Human
-
Immunogen
Recombinant full length protein within Human IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q14116 -
Positive control
- WB: PC-3, HeLa and HaCaT whole cell lysates. IHC-P: Human tonsil, liver and kidney tissues. Flow cyt: PC-3 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR19954-188
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab243091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 22, 14 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/50. |
Target
-
FunctionAugments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
-
Sequence similaritiesBelongs to the IL-1 family.
-
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 3606 Human
- Omim: 600953 Human
- SwissProt: Q14116 Human
- Unigene: 83077 Human
-
Alternative names
- Iboctadekin antibody
- IFN gamma inducing factor antibody
- IFN-gamma-inducing factor antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)](https://www.abcam.com/ps/products/243/ab243091/Images/ab243091-332569-anti-il-18-antibody-epr19954-188-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on distal convoluted tubules of human kidney (PMID: 17687255). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)](https://www.abcam.com/ps/products/243/ab243091/Images/ab243091-332568-anti-il-18-antibody-epr19954-188-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells in human liver (PMID: 19084941). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)](https://www.abcam.com/ps/products/243/ab243091/Images/ab243091-332567-anti-il-18-antibody-epr19954-188-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (ab243091)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on subsets of macrophages in tonsil (PMID: 28842466). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] (ab243091)](https://www.abcam.com/ps/products/243/ab243091/Images/ab243091-332566-anti-il-18-antibody-epr19954-188-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] (ab243091)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and PC-3 (human prostate adenocarcinoma cell line) (right panel) cells labeling IL-18 with ab243091 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat (PMID 15086390).
-
![Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)](https://www.abcam.com/ps/products/243/ab243091/Images/ab243091-332565-anti-il-18-antibody-epr19954-188-western-blot.jpg)
Western blot - Anti-IL-18 antibody [EPR19954-188] (ab243091)All lanes : Anti-IL-18 antibody [EPR19954-188] (ab243091) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 14,22 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 & 2: 3 minutes. Lane 3: 26 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
The bands observed are consistent with what has been described in the literature. Mature IL-18 protein is detected as a band at 18 kDa, whereas the 14-kDa band represents
the inactive form (PMID: 15326478).
Datasheets and documents
References
ab243091 has not yet been referenced specifically in any publications.
