Recombinant
RabMAb

Recombinant Anti-IL-18 antibody [EPR19954-188] (ab243091)

Overview

  • Product name
    Anti-IL-18 antibody [EPR19954-188]
    See all IL-18 primary antibodies
  • Description
    Rabbit monoclonal [EPR19954-188] to IL-18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14116

  • Positive control
    • WB: PC-3, HeLa and HaCaT whole cell lysates. IHC-P: Human tonsil, liver and kidney tissues. Flow cyt: PC-3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22, 14 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
    • Sequence similarities
      Belongs to the IL-1 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Iboctadekin antibody
      • IFN gamma inducing factor antibody
      • IFN-gamma-inducing factor antibody
      • IGIF antibody
      • IL 1 gamma antibody
      • IL 18 antibody
      • IL 1g antibody
      • IL-1 gamma antibody
      • IL-18 antibody
      • IL1 gamma antibody
      • IL18 antibody
      • IL18 protein antibody
      • IL18_HUMAN antibody
      • IL1F4 antibody
      • IL1g antibody
      • IL1gamma antibody
      • ILIF4 antibody
      • Interferon gamma inducing factor antibody
      • Interferon gamma-inducing factor antibody
      • Interleukin 1 gamma antibody
      • Interleukin 18 (interferon-gamma-inducing factor) antibody
      • Interleukin 18 antibody
      • Interleukin-1 gamma antibody
      • Interleukin-18 antibody
      • Interleukin18 antibody
      • MGC12320 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on distal convoluted tubules of human kidney (PMID: 17687255). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells in human liver (PMID: 19084941). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on subsets of macrophages in tonsil (PMID: 28842466). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and PC-3 (human prostate adenocarcinoma cell line) (right panel) cells labeling IL-18 with ab243091 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Jurkat (PMID 15086390).

    • All lanes : Anti-IL-18 antibody [EPR19954-188] (ab243091) at 1/1000 dilution

      Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 22 kDa
      Observed band size: 14,22 kDa
      why is the actual band size different from the predicted?



      Exposure time :  Lanes 1 & 2: 3 minutes. Lane 3: 26 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST. 

      The bands observed are consistent with what has been described in the literature. Mature IL-18 protein is detected as a band at 18 kDa, whereas the 14-kDa band represents
      the inactive form (PMID: 15326478).

    References

    ab243091 has not yet been referenced specifically in any publications.

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