Recombinant Anti-IL-18 antibody [EPR19956] (ab207323)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19956] to IL-18
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-18 antibody [EPR19956]
See all IL-18 primary antibodies -
Description
Rabbit monoclonal [EPR19956] to IL-18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse IL-18 active protein; Mouse spleen lysate. IP: Mouse spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19956 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IP |
1/40.
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Notes |
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WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IP
1/40. |
Target
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Function
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16173 Mouse
- SwissProt: P70380 Mouse
- Unigene: 1410 Mouse
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Alternative names
- Iboctadekin antibody
- IFN gamma inducing factor antibody
- IFN-gamma-inducing factor antibody
see all
Images
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Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution + Mouse IL-18 active protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution + Mouse spleen lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The protein expression level can refer to reference PMID 10051628.
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IL-18 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab207323 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse spleen lysate 10µg (Input).
Lane 2: ab207323 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207323 in mouse spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (23)
ab207323 has been referenced in 23 publications.
- Li H et al. Interleukin-18 mediated inflammatory brain injury after intracerebral hemorrhage in male mice. J Neurosci Res 100:1359-1369 (2022). PubMed: 35316547
- Yang J et al. An Experimental Study Reveals the Protective Effect of Autophagy against Realgar-Induced Liver Injury via Suppressing ROS-Mediated NLRP3 Inflammasome Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35628508
- Lv H et al. Exercise Preconditioning Ameliorates Cognitive Impairment in Mice with Ischemic Stroke by Alleviating Inflammation and Modulating Gut Microbiota. Mediators Inflamm 2022:2124230 (2022). PubMed: 36262547
- Dong D et al. VX-765 Alleviates β-Amyloid Deposition and Secondary Degeneration in the Ipsilateral Hippocampus and Ameliorates Cognitive Decline after Focal Cortical Infarction in Rats. J Mol Neurosci 72:2389-2397 (2022). PubMed: 36441377
- Liu N et al. TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. J Neuroinflammation 19:26 (2022). PubMed: 35093118