Recombinant
RabMAb

Recombinant Anti-IL-18 antibody [EPR22249-212] (ab223293)

Overview

  • Product name

    Anti-IL-18 antibody [EPR22249-212]
    See all IL-18 primary antibodies
  • Description

    Rabbit monoclonal [EPR22249-212] to IL-18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein within Rat IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P97636

  • Positive control

    • IHC-P: Rat and mouse lung and liver tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    • Function

      Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Iboctadekin antibody
      • IFN gamma inducing factor antibody
      • IFN-gamma-inducing factor antibody
      • IGIF antibody
      • IL 1 gamma antibody
      • IL 18 antibody
      • IL 1g antibody
      • IL-1 gamma antibody
      • IL-18 antibody
      • IL1 gamma antibody
      • IL18 antibody
      • IL18 protein antibody
      • IL18_HUMAN antibody
      • IL1F4 antibody
      • IL1g antibody
      • IL1gamma antibody
      • ILIF4 antibody
      • Interferon gamma inducing factor antibody
      • Interferon gamma-inducing factor antibody
      • Interleukin 1 gamma antibody
      • Interleukin 18 (interferon-gamma-inducing factor) antibody
      • Interleukin 18 antibody
      • Interleukin-1 gamma antibody
      • Interleukin-18 antibody
      • Interleukin18 antibody
      • MGC12320 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of mouse liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of mouse lung (PMID: 17989120). Countertsained with Heamtoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of rat liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of rat lung (PMID: 17989120). Countertsained with Heamtoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    References

    ab223293 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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