Recombinant Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
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Overview
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Product name
Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free
See all IL-18 primary antibodies -
Description
Rabbit monoclonal [EPR22249-212] to IL-18 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein within Rat IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P97636 -
Positive control
- IHC-P: Rat and mouse lung and liver tissues.
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General notes
Ab242022 is the carrier-free version of ab223293. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab242022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22249-212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab242022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16173 Mouse
- Entrez Gene: 29197 Rat
- SwissProt: P70380 Mouse
- SwissProt: P97636 Rat
- Unigene: 1410 Mouse
- Unigene: 11118 Rat
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Alternative names
- Iboctadekin antibody
- IFN gamma inducing factor antibody
- IFN-gamma-inducing factor antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)](https://www.abcam.com/ps/products/242/ab242022/Images/ab242022-327994-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of mouse liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)](https://www.abcam.com/ps/products/242/ab242022/Images/ab242022-327993-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of mouse lung (PMID: 17989120). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)](https://www.abcam.com/ps/products/242/ab242022/Images/ab242022-327992-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of rat liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)](https://www.abcam.com/ps/products/242/ab242022/Images/ab242022-327991-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of rat lung (PMID: 17989120). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).
Datasheets and documents
References
ab242022 has not yet been referenced specifically in any publications.
