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RecombinantRabMAb

Recombinant Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
  • Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22249-212] to IL-18 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

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Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Recombinant Human IL-18 protein (ab188059)

View more associated products

Overview

  • Product name

    Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free
    See all IL-18 primary antibodies
  • Description

    Rabbit monoclonal [EPR22249-212] to IL-18 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein within Rat IL-18 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P97636

  • Positive control

    • IHC-P: Rat and mouse lung and liver tissues.
  • General notes

    Ab242022 is the carrier-free version of ab223293. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22249-212
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-IL-18 antibody [EPR22249-212] (ab223293)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IL-18 protein (ab188059)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

Our Abpromise guarantee covers the use of ab242022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    • Function

      Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q14116 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 16173 Mouse
      • Entrez Gene: 29197 Rat
      • SwissProt: P70380 Mouse
      • SwissProt: P97636 Rat
      • Unigene: 1410 Mouse
      • Unigene: 11118 Rat
      • Alternative names

        • Iboctadekin antibody
        • IFN gamma inducing factor antibody
        • IFN-gamma-inducing factor antibody
        • IGIF antibody
        • IL 1 gamma antibody
        • IL 18 antibody
        • IL 1g antibody
        • IL-1 gamma antibody
        • IL-18 antibody
        • IL1 gamma antibody
        • IL18 antibody
        • IL18 protein antibody
        • IL18_HUMAN antibody
        • IL1F4 antibody
        • IL1g antibody
        • IL1gamma antibody
        • ILIF4 antibody
        • Interferon gamma inducing factor antibody
        • Interferon gamma-inducing factor antibody
        • Interleukin 1 gamma antibody
        • Interleukin 18 (interferon-gamma-inducing factor) antibody
        • Interleukin 18 antibody
        • Interleukin-1 gamma antibody
        • Interleukin-18 antibody
        • Interleukin18 antibody
        • MGC12320 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

        Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of mouse liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of mouse lung (PMID: 17989120). Countertsained with Heamtoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

        Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of rat liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

        Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of rat lung (PMID: 17989120). Countertsained with Heamtoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223293).

      • Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)
        Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (ab242022)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab242022? Please let us know so that we can cite the reference in this datasheet.

      ab242022 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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