Overview

  • Product name

    Anti-IL-18R Beta/IL-18RAP antibody
    See all IL-18R Beta/IL-18RAP primary antibodies
  • Description

    Rabbit polyclonal to IL-18R Beta/IL-18RAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IL-18R Beta/IL-18RAP aa 20-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    F NISGCSTKKL LWTYSTRSEE EFVLFCDLPE


    Database link: O95256

  • Positive control

    • Human pulmonitis tissue. Mouse cells.
  • General notes

     This product was previously labelled as IL-18R Beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab203191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 68 kDa.
Flow Cyt 1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
  • Tissue specificity

    Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • IL18RAP antibody
    • Accessory protein like antibody
    • Accessory protein-like antibody
    • AcPL antibody
    • CD218 antigen-like family member B antibody
    • CD218b antibody
    • CDw218b antibody
    • I18RA_HUMAN antibody
    • IL 18RAcP antibody
    • IL 1R accessory protein like antibody
    • IL 1R7 antibody
    • IL 1RAcPL antibody
    • IL-18 receptor accessory protein antibody
    • IL-18R-beta antibody
    • IL-18RAcP antibody
    • IL-18Rbeta antibody
    • IL-1R accessory protein-like antibody
    • IL-1R-7 antibody
    • IL-1R7 antibody
    • IL-1RAcPL antibody
    • IL18R Beta antibody
    • IL18RAP antibody
    • IL18Rbeta antibody
    • Interleukin 18 receptor accessory protein antibody
    • Interleukin-1 receptor 7 antibody
    • Interleukin-18 receptor accessory protein antibody
    • Interleukin-18 receptor accessory protein-like antibody
    • Interleukin-18 receptor beta antibody
    • Interleukin-18 receptor beta chain antibody
    see all

Images

  • Flow cytometry analysis of mouse cells labeling IL18R Beta (green, right histogram), using ab203191 at a 1/100 dilution, and negative control cells (blue, left histogram). Anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pulmonitis tissue, labeling IL18R Beta using ab203191 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab203191 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Feb 03 2017

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