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Immunology Innate Immunity Cytokines Interleukins
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RecombinantRabMAb

Recombinant Anti-IL-1RA antibody [EPR6483] (ab124962)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Immunoprecipitation - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Anti-IL-1RA antibody [EPR6483] (ab124962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6483] to IL-1RA
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-IL-1RA antibody [EPR6483]
    See all IL-1RA primary antibodies
  • Description

    Rabbit monoclonal [EPR6483] to IL-1RA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human IL-1RA aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P18510

  • Positive control

    • WB: A431, 293T, MOLT4, HeLa, RAW264.7, NIH/3T3, and L6 cell lysates. IHC-P: Human, mouse and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: A431 and HeLa cells. IP: NIH/3T3 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 8.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6483
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Alternative Versions

    • Biotin Anti-IL-1RA antibody [EPR6483] (ab205855)
    • Anti-IL-1RA antibody [EPR6483] - BSA and Azide free (ab226101)
    • Alexa Fluor® 488 Anti-IL-1RA antibody [EPR6483] (ab252007)
    • Alexa Fluor® 647 Anti-IL-1RA antibody [EPR6483] (ab252008)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 cytoplasmic extract lysate (ab14873)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human IL-1RA protein (ab50081)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
Rat
Human
IP
Mouse
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/500.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/500.

Target

  • Function

    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificity

    The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in disease

    Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • Cellular localization

    Cytoplasm and Secreted.
  • Target information above from: UniProt accession P18510 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3557 Human
    • Entrez Gene: 16181 Mouse
    • Entrez Gene: 60582 Rat
    • Omim: 147679 Human
    • SwissProt: P18510 Human
    • SwissProt: P25085 Mouse
    • SwissProt: P25086 Rat
    • Unigene: 81134 Human
    • Unigene: 882 Mouse
    • Unigene: 162640 Rat
    see all
  • Alternative names

    • DIRA antibody
    • F630041P17Rik antibody
    • ICIL 1RA antibody
    • ICIL-1RA antibody
    • ICIL1RA antibody
    • IL-1ra antibody
    • IL-1ra3 antibody
    • IL-1RN antibody
    • IL1 inhibitor antibody
    • IL1F3 antibody
    • IL1RA antibody
    • IL1RA_HUMAN antibody
    • IL1RN (IL1F3) antibody
    • IL1RN antibody
    • Interleukin 1 receptor antagonist antibody
    • Interleukin-1 receptor antagonist protein antibody
    • Intracellular IL 1 receptor antagonist type II antibody
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
    • IRAP antibody
    • MGC10430 antibody
    • MVCD4 antibody
    • Type II interleukin 1 receptor antagonist antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IL-1RA with unpurified ab124962 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124962 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling IL-1RA with purified ab124962 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Anti-IL-1RA antibody [EPR6483] (ab124962) at 20 µg (purified) + L6 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    All lanes : Anti-IL-1RA antibody [EPR6483] (ab124962) at 20 µg (purified)

    Lane 1 : A431 whole cell lysate
    Lane 2 : HEK293 whole cell lysate
    Lane 3 : Molt-4 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Raw264.7 whole cell lysate
    Lane 6 : NIH/3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL-1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling IL-1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IL-1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Flow Cytometry analysis of HeLa cells labelling IL-1RA with purified ab124962 at 1/20 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Immunoprecipitation - Anti-IL-1RA antibody [EPR6483] (ab124962)

    ab124962 (purified) at 1/20 immunoprecipitating IL-1RA in NIH/3T3 whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

    Lane 2 (+): ab124962 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124962 in NIH/3T3 whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
    All lanes : Anti-IL-1RA antibody [EPR6483] (ab124962) at 1/10000 dilution (unpurified)

    Lane 1 : A431 cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : MOLT4 cell lysates
    Lane 4 : HeLa cell lysates
    Lane 5 : RAW264.7 cell lysates
    Lane 6 : NIH 3T3 cell lysates
    Lane 7 : L6 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 20 kDa

  • Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Flow Cytometry - Anti-IL-1RA antibody [EPR6483] (ab124962)

    Overlay histogram showing A431 cells stained with unpurified ab124962 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab124962, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
    OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IL-1RA antibody [EPR6483] (ab124962)
    Anti-IL-1RA antibody [EPR6483] (ab124962)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (15)

    Publishing research using ab124962? Please let us know so that we can cite the reference in this datasheet.

    ab124962 has been referenced in 15 publications.

    • He W  et al. Microglial NLRP3 inflammasome activation mediates IL-1ß release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine model. J Neuroinflammation 16:78 (2019). PubMed: 30971286
    • Wang K  et al. IL1RN mediates the suppressive effect of methionine deprivation on glioma proliferation. Cancer Lett 454:146-157 (2019). PubMed: 30978442
    • Vander Beken S  et al. Newly Defined ATP-Binding Cassette Subfamily B Member 5 Positive Dermal Mesenchymal Stem Cells Promote Healing of Chronic Iron-Overload Wounds via Secretion of Interleukin-1 Receptor Antagonist. Stem Cells 37:1057-1074 (2019). PubMed: 31002437
    • Ackerman JE  et al. Cell non-autonomous functions of S100a4 drive fibrotic tendon healing. Elife 8:N/A (2019). PubMed: 31124787
    • Xu B  et al. Increased AIF-1-mediated TNF-a expression during implantation phase in IVF cycles with GnRH antagonist protocol. Hum Reprod 33:1270-1280 (2018). PubMed: 29897458
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (rat ligament)
    Permeabilization
    No
    Specification
    rat ligament
    Blocking step
    Biocare Rodent Block R as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted May 28 2014

    ELISA abreview for Anti-IL-1RA antibody [EPR6483]

    Poor
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
    Specification
    St. protein from Peprotech ELISA development kit ,
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Type
    Sandwich (Capture)
    Read More

    Mr. Toomas Neuman

    Verified customer

    Submitted Jan 29 2014

    ELISA abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
    Specification
    St. protein from Peprotech ELISA development kit ,
    Read More

    Mr. Toomas Neuman

    Verified customer

    Submitted Jan 29 2014

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