Recombinant Anti-IL-1RA antibody [EPR6483] (ab124962)
![Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)](https://www.abcam.com/ps/products/124/ab124962/Images/ab124962-472348-anti-il-1ra-antibody-epr6483-western-blot-wildtype-a431-il1rail1rn-knockout-a431.jpg "Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6483] to IL-1RA
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-1RA antibody [EPR6483]
See all IL-1RA primary antibodies -
Description
Rabbit monoclonal [EPR6483] to IL-1RA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-1RA aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P18510 -
Positive control
- WB: A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 8.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6483 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Observed band at >20 kDa is likely an unrelated protein. |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Observed band at >20 kDa is likely an unrelated protein. |
Target
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Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
Tissue specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
Involvement in disease
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
Cellular localization
Cytoplasm and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3557 Human
- Omim: 147679 Human
- SwissProt: P18510 Human
- Unigene: 81134 Human
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Alternative names
- DIRA antibody
- F630041P17Rik antibody
- ICIL 1RA antibody
see all
Images
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Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)All lanes : Anti-IL-1RA antibody [EPR6483] (ab124962) at 1/10000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : IL-1RA knockout A431 cell lysate
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-IL-1RA antibody [EPR6483] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124962 was shown to bind specifically to IL-1RA. A band was observed at 19 kDa in wild-type A431 cell lysates with no signal observed at this size in IL1RN knockout cell line ab273379 (knockout cell lysate ab275530). To generate this image, wild-type and IL1RN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)](https://www.abcam.com/ps/products/124/ab124962/Images/ab124962-139033-anti-il-1ra-antibody-epr6483.jpg)
OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962) -
![Anti-IL-1RA antibody [EPR6483] (ab124962)](https://www.abcam.com/ps/products/124/ab124962/Images/ab124962-14-benefits-of-recombinant-antibodies.png)
Anti-IL-1RA antibody [EPR6483] (ab124962)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab124962 has been referenced in 23 publications.
- Zhang L et al. Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist. Theranostics 11:1147-1161 (2021). PubMed: 33391526
- Gajawada P et al. Myocardial Accumulations of Reg3A, Reg3? and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis. Int J Mol Sci 22:N/A (2021). PubMed: 33923774
- Chu LW et al. Loganin prevents chronic constriction injury-provoked neuropathic pain by reducing TNF-a/IL-1ß-mediated NF-?B activation and Schwann cell demyelination. Phytomedicine 67:153166 (2020). PubMed: 31955133
- Peng YJ et al. Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1a/IL-1 Receptor Antagonist Pathway. Mediators Inflamm 2020:1237281 (2020). PubMed: 32587467
- Liu J et al. Inhibition of GSK3ß and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production. Front Pharmacol 11:812 (2020). PubMed: 32595496