Anti-IL-1RAcP antibody (ab232950)
Key features and details
- Rabbit polyclonal to IL-1RAcP
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-IL-1RAcP antibody
See all IL-1RAcP primary antibodies -
Description
Rabbit polyclonal to IL-1RAcP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment (His-tag) corresponding to Human IL-1RAcP aa 50-250. (Expressed in E.coli).
Database link: Q9NPH3 -
Positive control
- IHC-P: Human lung tissue. WB: Cow thymus lysate; Recombinant human IL-1RAcP protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232950 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 65 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 65 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. -
Tissue specificity
Detected in liver, skin, placenta, thymus and lung. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 539334 Cow
- Entrez Gene: 3556 Human
- Entrez Gene: 574387 Rhesus monkey
- Omim: 602626 Human
- SwissProt: Q9NPH3 Human
- SwissProt: P59822 Rhesus monkey
- Unigene: 478673 Human
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Alternative names
- C3orf13 antibody
- FLJ37788 antibody
- IL 1 receptor accessory protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for IL-1RAcP using ab232950 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-IL-1RAcP antibody (ab232950) at 2 µg/ml + Recombinant human IL-1RAcP protein
Predicted band size: 65 kDa -
Anti-IL-1RAcP antibody (ab232950) at 2 µg/ml + Cow thymus tissue lysate.
Predicted band size: 65 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232950 has not yet been referenced specifically in any publications.