Key features and details
- Rabbit polyclonal to IL-1RAcP
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
Product nameAnti-IL-1RAcP antibody
See all IL-1RAcP primary antibodies
DescriptionRabbit polyclonal to IL-1RAcP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Rhesus monkey
Recombinant fragment (His-tag) corresponding to Human IL-1RAcP aa 65-249. (Expressed in E.coli).
GLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTC MLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVD GYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVV TYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPN
Database link: Q9NPH3
- IHC-P: Human lung tissue. WB: Cow thymus lysate; Recombinant human IL-1RAcP protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232950 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 65 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCoreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Tissue specificityDetected in liver, skin, placenta, thymus and lung.
Sequence similaritiesBelongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- C3orf13 antibody
- FLJ37788 antibody
- IL 1 receptor accessory protein antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for IL-1RAcP using ab232950 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-IL-1RAcP antibody (ab232950) at 2 µg/ml + Recombinant human IL-1RAcP protein
Predicted band size: 65 kDa
Anti-IL-1RAcP antibody (ab232950) at 2 µg/ml + Cow thymus tissue lysate.
Predicted band size: 65 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232950 has not yet been referenced specifically in any publications.