Anti-IL-1RAcP antibody (ab8110)
Key features and details
- Rabbit polyclonal to IL-1RAcP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-1RAcP antibody
See all IL-1RAcP primary antibodies -
Description
Rabbit polyclonal to IL-1RAcP -
Host species
Rabbit -
Specificity
It has no cross activity to other members in the IL-1 receptor family. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human IL-1RAcP aa 525-540. sequence is identical to those of mouse and rat origins
(Peptide available asab6104) -
Positive control
- WB: HeLa whole cell lysate ICC/IF: HeLa cells. IF: HeLa cells.
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General notes
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab8110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/2000. Detects a band of approximately 66 kDa. | |
ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. -
Tissue specificity
Detected in liver, skin, placenta, thymus and lung. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3556 Human
- Entrez Gene: 16180 Mouse
- Entrez Gene: 25466 Rat
- Omim: 602626 Human
- SwissProt: Q9NPH3 Human
- SwissProt: Q61730 Mouse
- SwissProt: Q63621 Rat
- Unigene: 478673 Human
see all -
Alternative names
- C3orf13 antibody
- FLJ37788 antibody
- IL 1 receptor accessory protein antibody
see all
Images
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Immunocytochemistry of HeLa cells labeling IL-1RAcP using ab8110 at 2 µg/ml.
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Anti-IL-1RAcP antibody (ab8110) at 1/1000 dilution + HeLa whole cell lysate
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence of HeLa cells staining IL-1RAcP with ab8110 at 5 µg/ml
Protocols
Datasheets and documents
References (9)
ab8110 has been referenced in 9 publications.
- Wang N et al. Potential regulatory network in the PSG10P/miR-19a-3p/IL1RAP pathway is possibly involved in preeclampsia pathogenesis. J Cell Mol Med 23:852-864 (2019). PubMed: 30370628
- Arnold ND et al. A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension. Nat Commun 10:5183 (2019). PubMed: 31729368
- Zhang WJ et al. Ginsenoside Rd inhibits IL-1ß-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination. Braz J Med Biol Res 52:e8525 (2019). PubMed: 31411316
- Liang Y et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). PubMed: 30410547
- Theodoropoulou S et al. Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye. J Pathol 241:45-56 (2017). PubMed: 27701734
- Wan X et al. Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing. Sci Rep 6:36589 (2016). WB, IHC . PubMed: 27833113
- Nadeau-Vallée M et al. Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth. J Immunol 195:3402-15 (2015). WB ; Mouse . PubMed: 26304990
- Planck SR et al. Impact of IL-1 signalling on experimental uveitis and arthritis. Ann Rheum Dis 71:753-60 (2012). ICC/IF ; Mouse . PubMed: 22267332
- Kim HS et al. Identification of novel Wilms' tumor suppressor gene target genes implicated in kidney development. J Biol Chem 282:16278-87 (2007). IHC-P ; Rat . PubMed: 17430890