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    il-1racp-antibody-ab8110.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-1RAcP antibody (ab8110)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)
  • Western blot - Anti-IL-1RAcP antibody (ab8110)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)

Key features and details

  • Rabbit polyclonal to IL-1RAcP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IL-1RAcP antibody
    See all IL-1RAcP primary antibodies
  • Description

    Rabbit polyclonal to IL-1RAcP
  • Host species

    Rabbit
  • Specificity

    It has no cross activity to other members in the IL-1 receptor family.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IL-1RAcP aa 525-540. sequence is identical to those of mouse and rat origins
    (Peptide available as ab6104)

  • Positive control

    • WB: HeLa whole cell lysate ICC/IF: HeLa cells. IF: HeLa cells.
  • General notes


    The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IL-1RAcP protein (ab157075)

Applications

Our Abpromise guarantee covers the use of ab8110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
  • Tissue specificity

    Detected in liver, skin, placenta, thymus and lung.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9NPH3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3556 Human
    • Entrez Gene: 16180 Mouse
    • Entrez Gene: 25466 Rat
    • Omim: 602626 Human
    • SwissProt: Q9NPH3 Human
    • SwissProt: Q61730 Mouse
    • SwissProt: Q63621 Rat
    • Unigene: 478673 Human
    • Unigene: 253424 Mouse
    • Unigene: 10511 Rat
    see all
  • Alternative names

    • C3orf13 antibody
    • FLJ37788 antibody
    • IL 1 receptor accessory protein antibody
    • IL 1R 3 antibody
    • IL 1R3 antibody
    • IL 1RAcP antibody
    • IL 1RAP antibody
    • IL-1 receptor accessory protein antibody
    • IL-1R-3 antibody
    • IL-1R3 antibody
    • IL-1RAcP antibody
    • IL-1RAP antibody
    • IL1 receptor accessory protein antibody
    • IL1AP_HUMAN antibody
    • IL1R3 antibody
    • IL1RAcP antibody
    • Il1rap antibody
    • Interleukin 1 Accessory Protein antibody
    • interleukin 1 receptor 3 antibody
    • Interleukin 1 receptor accessory protein antibody
    • interleukin 1 receptor accessory protein beta antibody
    • Interleukin-1 receptor 3 antibody
    • Interleukin-1 receptor accessory protein antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)

    Immunocytochemistry of HeLa cells labeling IL-1RAcP using ab8110 at 2 µg/ml. 

  • Western blot - Anti-IL-1RAcP antibody (ab8110)
    Western blot - Anti-IL-1RAcP antibody (ab8110)
    Anti-IL-1RAcP antibody (ab8110) at 1/1000 dilution + HeLa whole cell lysate

    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-1RAcP antibody (ab8110)

    Immunocytochemistry/ Immunofluorescence of HeLa cells staining IL-1RAcP with ab8110 at 5 µg/ml

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab8110? Please let us know so that we can cite the reference in this datasheet.

    ab8110 has been referenced in 9 publications.

    • Wang N  et al. Potential regulatory network in the PSG10P/miR-19a-3p/IL1RAP pathway is possibly involved in preeclampsia pathogenesis. J Cell Mol Med 23:852-864 (2019). PubMed: 30370628
    • Arnold ND  et al. A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension. Nat Commun 10:5183 (2019). PubMed: 31729368
    • Zhang WJ  et al. Ginsenoside Rd inhibits IL-1ß-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination. Braz J Med Biol Res 52:e8525 (2019). PubMed: 31411316
    • Liang Y  et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). PubMed: 30410547
    • Theodoropoulou S  et al. Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye. J Pathol 241:45-56 (2017). PubMed: 27701734
    • Wan X  et al. Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing. Sci Rep 6:36589 (2016). WB, IHC . PubMed: 27833113
    • Nadeau-Vallée M  et al. Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth. J Immunol 195:3402-15 (2015). WB ; Mouse . PubMed: 26304990
    • Planck SR  et al. Impact of IL-1 signalling on experimental uveitis and arthritis. Ann Rheum Dis 71:753-60 (2012). ICC/IF ; Mouse . PubMed: 22267332
    • Kim HS  et al. Identification of novel Wilms' tumor suppressor gene target genes implicated in kidney development. J Biol Chem 282:16278-87 (2007). IHC-P ; Rat . PubMed: 17430890

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