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  1. Link

    il-1racp-antibody-epr22678-67-ab256461.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • ELISA - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
  • Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22678-67] to IL-1RAcP
  • Suitable for: ELISA, WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Recombinant Human IL-1RAcP protein (ab151831)
Knockout
Product image
Human IL1RAP knockout HeLa cell line (ab273375)

View more associated products

Overview

  • Product name

    Anti-IL-1RAcP antibody [EPR22678-67]
    See all IL-1RAcP primary antibodies
  • Description

    Rabbit monoclonal [EPR22678-67] to IL-1RAcP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: KARPAS-299 and HepG2 whole cell lysate. Flow Cyt: HeLa and HepG2 cells. IP: KARPAS-299 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22678-67
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Alternative Versions

    • Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human IL1RAP knockout HeLa cell line (ab273375)
  • Recombinant Protein

    • Recombinant Human IL-1RAcP protein (ab151831)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa).
Flow Cyt
1/60.
IP
1/30.
Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa).
Flow Cyt
1/60.
IP
1/30.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
  • Tissue specificity

    Detected in liver, skin, placenta, thymus and lung.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9NPH3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3556 Human
    • Omim: 602626 Human
    • SwissProt: Q9NPH3 Human
    • Unigene: 478673 Human
    • Alternative names

      • C3orf13 antibody
      • FLJ37788 antibody
      • IL 1 receptor accessory protein antibody
      • IL 1R 3 antibody
      • IL 1R3 antibody
      • IL 1RAcP antibody
      • IL 1RAP antibody
      • IL-1 receptor accessory protein antibody
      • IL-1R-3 antibody
      • IL-1R3 antibody
      • IL-1RAcP antibody
      • IL-1RAP antibody
      • IL1 receptor accessory protein antibody
      • IL1AP_HUMAN antibody
      • IL1R3 antibody
      • IL1RAcP antibody
      • Il1rap antibody
      • Interleukin 1 Accessory Protein antibody
      • interleukin 1 receptor 3 antibody
      • Interleukin 1 receptor accessory protein antibody
      • interleukin 1 receptor accessory protein beta antibody
      • Interleukin-1 receptor 3 antibody
      • Interleukin-1 receptor accessory protein antibody
      see all

    Images

    • Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

      Flow cytometry overlay histogram showing wild-type HeLa (green line) and IL1RAP knockout HeLa cells (ab273375) stained with ab256461 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab256461) (1x106 in 100μl at 10 μ/ml) for 30 min at 4°C.

      The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.

      Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line; IL1RAP knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

      Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    • Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      All lanes : Anti-IL-1RAcP antibody [EPR22678-67] (ab256461) at 1/1000 dilution

      Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
      Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature (PMID: 30514753).

      Negative control: Raji (PMID: 30514753).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Anti-IL-1RAcP antibody [EPR22678-67] (ab256461) at 1/1000 dilution + HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature (PMID: 30514753).

      This blot was developed using a higher sensitivity ECL substrate.

      Blocking/Dilution buffer: 5% NFDM/TBT.

    • Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

      Flow cytometric analysis of Raji (Human burkitt's lymphoma b lymphocyte, Left panel) and HepG2 (Human hepatocellular carcinoma epithelial cell, Right panel) cells labeling IL-1RAcP with ab256461 at a 1/60 dilution (1μg, red), compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 30514753). Gated on viable cells.

    • Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

      IL-1RAcP was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg with ab256461 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256461 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg.

      Lane 2: ab256461 IP in KARPAS-299 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256461 in KARPAS-299 whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 75 secs.

    • ELISA - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      ELISA - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      ELISA analysis of Hu IL1RAP recombinant protein at 1000 ng/mL with ab256461. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
      Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab256461? Please let us know so that we can cite the reference in this datasheet.

    ab256461 has not yet been referenced specifically in any publications.

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