Recombinant Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
![Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-7-Anti-IL-1RAc-EPR22678-67-Human-HeLa-cells-flow-cytometry.jpg "Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22678-67] to IL-1RAcP
- Suitable for: ELISA, WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-IL-1RAcP antibody [EPR22678-67]
See all IL-1RAcP primary antibodies -
Description
Rabbit monoclonal [EPR22678-67] to IL-1RAcP -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: KARPAS-299 and HepG2 whole cell lysate. Flow Cyt: HeLa and HepG2 cells. IP: KARPAS-299 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22678-67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa).
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| Flow Cyt |
1/60.
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| IP |
1/30.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa). |
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Flow Cyt
1/60. |
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IP
1/30. |
Target
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Function
Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. -
Tissue specificity
Detected in liver, skin, placenta, thymus and lung. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3556 Human
- Omim: 602626 Human
- SwissProt: Q9NPH3 Human
- Unigene: 478673 Human
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Alternative names
- C3orf13 antibody
- FLJ37788 antibody
- IL 1 receptor accessory protein antibody
see all
Images
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Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
Flow cytometry overlay histogram showing wild-type HeLa (green line) and IL1RAP knockout HeLa cells (ab273375) stained with ab256461 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab256461) (1x106 in 100μl at 10 μ/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line; IL1RAP knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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![Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-3-anti-il1racp-antibody-epr22678-67-western-blot-karpas299-human.jpg)
Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)All lanes : Anti-IL-1RAcP antibody [EPR22678-67] (ab256461) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID: 30514753).
Negative control: Raji (PMID: 30514753).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-352260-anti-il-1racp-antibody-western-blot-human-hepg2-cells.jpg)
Western blot - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)Anti-IL-1RAcP antibody [EPR22678-67] (ab256461) at 1/1000 dilution + HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID: 30514753).
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBT.
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![Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-1-anti-il1racp-antibody-epr22678-67-flowcytometry-hepg2-raji-human.jpg)
Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)Flow cytometric analysis of Raji (Human burkitt's lymphoma b lymphocyte, Left panel) and HepG2 (Human hepatocellular carcinoma epithelial cell, Right panel) cells labeling IL-1RAcP with ab256461 at a 1/60 dilution (1μg, red), compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 30514753). Gated on viable cells.
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![Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-2-anti-il1racp-antibody-epr22678-67-immunoprecipitation-karpas299-human.jpg)
Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)IL-1RAcP was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg with ab256461 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256461 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg.
Lane 2: ab256461 IP in KARPAS-299 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256461 in KARPAS-299 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 75 secs.
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![ELISA - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-5-anti-il-1racp-antibody-epr22678-67-elisa-huil1rap.jpg)
ELISA - Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)ELISA analysis of Hu IL1RAP recombinant protein at 1000 ng/mL with ab256461. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)](https://www.abcam.com/ps/products/256/ab256461/Images/ab256461-6-benefits-of-recombinant-antibodies.png)
Anti-IL-1RAcP antibody [EPR22678-67] (ab256461)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab256461 has not yet been referenced specifically in any publications.
