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    il-2-antibody-ab14036.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-2 antibody (ab14036)

  • Datasheet
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Abpromise

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Western blot - Anti-IL-2 antibody (ab14036)

    Key features and details

    • Chicken polyclonal to IL-2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgY

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Protein
    Recombinant mouse IL-2 protein (ab9856)

    View more associated products

    Overview

    • Product name

      Anti-IL-2 antibody
      See all IL-2 primary antibodies
    • Description

      Chicken polyclonal to IL-2
    • Host species

      Chicken
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to IL-2 aa 21-153.

    • Positive control

      • Purchase matching WB positive control:Recombinant human IL-2 protein
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgY
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins

    Associated products

    • Isotype control

      • Chicken IgY - Isotype Control (ab50579)
    • Recombinant Protein

      • Recombinant mouse IL-2 protein (ab9856)

    Applications

    Our Abpromise guarantee covers the use of ab14036 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
  • Application notes
    ELISA: Use at a concentration of 0.1 µg/ml.
    WB: 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

    This product has only been tested against the recombinant form of the protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • Involvement in disease

      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • Sequence similarities

      Belongs to the IL-2 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P60568 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3558 Human
      • Omim: 147680 Human
      • SwissProt: P60568 Human
      • Unigene: 89679 Human
      • Alternative names

        • Aldesleukin antibody
        • IL 2 antibody
        • IL-2 antibody
        • IL2 antibody
        • IL2_HUMAN antibody
        • Interleukin 2 antibody
        • Interleukin-2 antibody
        • interleukin2 antibody
        • Involved in regulation of T cell clonal expansion antibody
        • Lymphokine antibody
        • OTTHUMP00000164090 antibody
        • POIL2 antibody
        • T Cell Growth Factor antibody
        • T-cell growth factor antibody
        • TCGF antibody
        see all

      Images

      • Western blot - Anti-IL-2 antibody (ab14036)
        Western blot - Anti-IL-2 antibody (ab14036)

        Recombinant human IL2 as antigen. ab14036 dilution: 1/2000.

         

        Recombinant human IL2 as antigen. ab14036 dilution: 1/2000.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab14036? Please let us know so that we can cite the reference in this datasheet.

      ab14036 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Customer is searching for an IL2 antibody that cross-reacts with human and can be used in a sandwich ELISA as both a capture and detection antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 12 2005

      Answer

      Thank you for your enquiry. Ab9618 has only been tested in a direct ELISA, as follows: To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-2. As well, ab14036 has only been tested in a direct ELISA. Our source for this antibody did mention that based on their experience, the antibody could in theory be conjugated to HRP and used in a sandwich ELISA. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on Jul 14 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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