Anti-IL-2 antibody (ab14036)

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Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgY

  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab14036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at a concentration of 0.1 µg/ml.
    WB: 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

    This product has only been tested against the recombinant form of the protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

    • Involvement in disease

      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

    • Sequence similarities

      Belongs to the IL-2 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Aldesleukin antibody
        • IL 2 antibody
        • IL-2 antibody
        • IL2 antibody
        • IL2_HUMAN antibody
        • Interleukin 2 antibody
        • Interleukin-2 antibody
        • interleukin2 antibody
        • Involved in regulation of T cell clonal expansion antibody
        • Lymphokine antibody
        • OTTHUMP00000164090 antibody
        • POIL2 antibody
        • T Cell Growth Factor antibody
        • T-cell growth factor antibody
        • TCGF antibody
        see all

      Images

      • Western blot - Anti-IL-2 antibody (ab14036)
        Western blot - Anti-IL-2 antibody (ab14036)

        Recombinant human IL2 as antigen. ab14036 dilution: 1/2000.

         

        Recombinant human IL2 as antigen. ab14036 dilution: 1/2000.

      References

      ab14036 has not yet been referenced specifically in any publications.

      Publishing research using ab14036? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Question

      Customer is searching for an IL2 antibody that cross-reacts with human and can be used in a sandwich ELISA as both a capture and detection antibody.

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      Answer

      Thank you for your enquiry. Ab9618 has only been tested in a direct ELISA, as follows: To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-2. As well, ab14036 has only been tested in a direct ELISA. Our source for this antibody did mention that based on their experience, the antibody could in theory be conjugated to HRP and used in a sandwich ELISA. If you have any additional questions, please contact us again.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com