Anti-IL-2 antibody (ab180780)

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Overview

  • Product name

    Anti-IL-2 antibody
    See all IL-2 primary antibodies

  • Description

    Rabbit polyclonal to IL-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein corresponding to Human IL-2 aa 1-153.
    Sequence:

    MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINN YKNPKLTRML TFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNF HLRPRDLISNINVIVLELKGSE TTFMCEYADETATIVEFLNRWITFCQ SIISTLT


    Database link: P60568

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • CTLL-2 cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

  • Sequence similarities

    Belongs to the IL-2 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • interleukin2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody (ab180780)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody (ab180780)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling IL2 with ab180780 at 1/100. Magnification: 200x.

    References

    ab180780 has not yet been referenced specifically in any publications.

    Publishing research using ab180780? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-IL-2 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (CT26.WT (ATCC® CRL-2638™) cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin Gel, PVDF membrane)
    Loading amount
    50 µg
    Specification
    CT26.WT (ATCC® CRL-2638™) cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 26 2019

    Immunocytochemistry abreview for Anti-IL-2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (CT26.WT (ATCC® CRL-2638™) cells)
    Permeabilization
    Yes - 0.1% triton-X100 in PBS
    Specification
    CT26.WT (ATCC® CRL-2638™) cells
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (EMT6 (ATCC® CRL-2755™) tumors taken out C57black m)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6 15min boiling
    Permeabilization
    No
    Specification
    EMT6 (ATCC® CRL-2755™) tumors taken out C57black m
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH:6 15min boiling
    Permeabilization
    No
    Specification
    MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 18 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (OV90 cells)
    Permeabilization
    Yes - 0.1% triton-X100 in PBS
    Specification
    OV90 cells
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2019

    Western blot abreview for Anti-IL-2 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT-29 (ATCC® HTB-38™))
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin Gel)
    Loading amount
    50 µg
    Specification
    HT-29 (ATCC® HTB-38™)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2019

    Immunocytochemistry abreview for Anti-IL-2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (OV90 cells)
    Permeabilization
    Yes - 0.1% triton-X100 in PBS
    Specification
    OV90 cells
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 16 2019

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