Key features and details
- Rabbit polyclonal to IL-2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-2 antibody
See all IL-2 primary antibodies
DescriptionRabbit polyclonal to IL-2
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein aa 21-153. The immunogen corresponds to the mature full length protein minus the signal peptide.
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT
Database link: P60568
- Purchase matching WB positive control:Recombinant human IL-2 protein
- Recombinant human IL2 protein (ab9619) can be used as a positive control in WB.
FormLyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
No preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich ELISA||Use at an assay dependent concentration.
To detect hIL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
Sequence similaritiesBelongs to the IL-2 family.
- Information by UniProt
- Aldesleukin antibody
- IL 2 antibody
- IL-2 antibody
ab9618 has been referenced in 2 publications.
- Kim Y et al. Fluorescence Turn-on Synthetic Lipid Rafts on Supramolecular Sheets and Hierarchical Concanavalin A Assembly. Chem Asian J 14:952-957 (2019). PubMed: 30779325
- LaMonica BE et al. Mitotic spindle orientation predicts outer radial glial cell generation in human neocortex. Nat Commun 4:1665 (2013). PubMed: 23575669