Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIL-2 (mouse Interleukin-2)

  • Positive control

    • Recombinant mouse IL2 protein (ab9856) can be used as a positive control in WB.

Properties

  • Form

    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (2.0 ng/ml), a concentration of 0.04 - 0.05 µg/ml of this antibody is required.

    ELISA:To detect mIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-2.

    Western Blot:To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight in Human: 15 kDa.
  • Target

    • Function

      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • Involvement in disease

      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • Sequence similarities

      Belongs to the IL-2 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • interleukin2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    References

    ab9855 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Murine IL-2 has a molecular weight of approx. 17.2 kDa but due to O-linked glycosilation may run slightly higher. We are not aware of any reports in which a 30kDa band is detected for Murine IL2 from mouse tissues. Have you tried running recombinant Murine IL2 as a positive control?

    Read More

    Answer

    Thank you for your enquiry. The molecular weight of Human IL-2 is 15.2kDa. May I ask if you are using human IL-2 standard? We generally see additional bands at higher molecular weights when loading proteins at higher concentrations. If you send us a picture of your Western blot (with the concentrations of antigen loaded and the dilution of the primary antibody used), we can take a look at it and see how it compares to the Westerns that we have run.

    Read More

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