Overview

  • Product name

    Anti-IL-2 antibody [EPR2780]
    See all IL-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2780] to IL-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human thymus and PBMC cell lysates, Human tonsil tissue; Jurkat cell lydate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2780
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • Involvement in disease

      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • Sequence similarities

      Belongs to the IL-2 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • interleukin2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    Images

    • ab92381 at 1/250 dilution staining IL2 in Formalin-fixed, Paraffin-embedded Human tonsil tissue. Detection: DAB staining.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • All lanes : Anti-IL-2 antibody [EPR2780] (ab92381) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : PBMC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa

    • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IL2 with purified ab92381 at 1/100 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    References

    This product has been referenced in:

    • Kaewkangsadan V  et al. Tumour-draining axillary lymph nodes in patients with large and locally advanced breast cancers undergoing neoadjuvant chemotherapy (NAC): the crucial contribution of immune cells (effector, regulatory) and cytokines (Th1, Th2) to immune-mediated tumour cell death induced by NAC. BMC Cancer 18:123 (2018). IHC-P ; Human . Read more (PubMed: 29390966) »
    • Voos P  et al. Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. Front Immunol 9:922 (2018). Read more (PubMed: 29760710) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (HT29 tumors taken out Balb/C nude mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph6
    Permeabilization
    No
    Specification
    HT29 tumors taken out Balb/C nude mice
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 15 2019

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (DLD-1 (ATCC® CCL-221™) cells)
    Permeabilization
    Yes - 0.1% triton-X100 in PBS
    Specification
    DLD-1 (ATCC® CCL-221™) cells
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 20 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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