Anti-IL-2 Receptor alpha antibody (ab235906)
Key features and details
- Rabbit polyclonal to IL-2 Receptor alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IL-2 Receptor alpha antibody
See all IL-2 Receptor alpha primary antibodies -
Description
Rabbit polyclonal to IL-2 Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment corresponding to Human IL-2 Receptor alpha aa 1-200.
Database link: P01589 -
Positive control
- WB: Rat spleen lysate. IHC-P: Human glioma tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/5000. Predicted molecular weight: 31 kDa.
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| IHC-P |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/5000. Predicted molecular weight: 31 kDa. |
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IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 450884 Chimpanzee
- Entrez Gene: 3559 Human
- Entrez Gene: 25704 Rat
- Entrez Gene: 574300 Rhesus monkey
- Omim: 147730 Human
- SwissProt: Q38IC8 Chimpanzee
- SwissProt: P01589 Human
- SwissProt: P26897 Rat
see all -
Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody (ab235906)
Paraffin-embedded human glioma tissue stained for IL2 Receptor alpha using ab235906 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-IL-2 Receptor alpha antibody (ab235906)Anti-IL-2 Receptor alpha antibody (ab235906) at 1/500 dilution + Rat spleen lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody (ab235906)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IL2 Receptor alpha (green) using ab235906 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235906 has not yet been referenced specifically in any publications.