Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [Basiliximab]
    See all IL-2 Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [Basiliximab] to IL-2 Receptor alpha
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit. Binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Tissue, cells or virus corresponding to IL-2 Receptor alpha. CTC 16 cells.

  • Positive control

    • IHC-P: Rat spleen and thymus tissue. Flow Cyt: Human, rhesus monkey and cynomolgus monkey lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab245687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for interleukin-2.
  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2 Receptor alpha antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Images

  • Formalin-fixed rat spleen tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis.

  • Formaldehyde-fixed rat thymus tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis. Detected with an FITC-conjugated secondary antibody.

  • Human lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

  • Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

  • Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

References

ab245687 has not yet been referenced specifically in any publications.

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