Recombinant
RabMAb

Recombinant Anti-IL-2 Receptor alpha antibody [EPR22588-18] (ab227834)

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [EPR22588-18]
    See all IL-2 Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR22588-18] to IL-2 Receptor alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse IL-2 Receptor alpha aa 100-200. The exact sequence is proprietary.
    Database link: P01590

  • Positive control

    • IHC-P: Mouse and rat spleen tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IP or WB.
  • Target

    • Function

      Receptor for interleukin-2.
    • Involvement in disease

      Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similarities

      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2 Receptor alpha antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of mouse spleen (PMID:17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of rat spleen (PMID:17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    References

    ab227834 has not yet been referenced specifically in any publications.

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