Recombinant
RabMAb

Recombinant Anti-IL-2 Receptor alpha antibody [EPR6452] (Alexa Fluor® 647) (ab205859)

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [EPR6452] (Alexa Fluor® 647)
    See all IL-2 Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR6452] to IL-2 Receptor alpha (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IL-2 Receptor alpha.
    Database link: P01589

  • Positive control

    • ICC/IF: K562 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Receptor for interleukin-2.
  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2 Receptor alpha antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Images

  • ab205859 staining IL2 Receptor in K562 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205859 at 1/100 dilution (shown in red). Cell membranes were labelled with WGA (Alexa Fluor® 488, shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in K562 cells fixed with 4% formaldehyde (10 min).

References

ab205859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205859.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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