Recombinant
RabMAb

Recombinant Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free (ab215378)

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free
    See all IL-2 Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR6452] to IL-2 Receptor alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IL-2 Receptor alpha aa 1-250.
    Database link: P01589

  • Positive control

    • IHC-P: Human lung carcinoma, Hodgkin's lymphoma, human tonsil, spleen, liver, and thymus tissues. ICC/IF: Jurkat cells.
  • General notes

    Ab215378 is the carrier-free version of ab128955. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab215378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Receptor for interleukin-2.
  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2 Receptor alpha antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human Hodgkin’s lymphoma tissue labeling Anti-IL-2 Receptor alpha with ab215378 at 1/250 dilution (10 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin’s lymphoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunofluorescence staining of Jurkat cells with purified ab128955 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab128955 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Anti-IL-2 Receptor alpha with ab215378 at 1/250 dilution (10 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on stromal cells in human lung carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Immunohistochemical staining of paraffin embedded human Hodgkin lymphoma with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Unpurified ab128955, at 1/250 dilution, staining IL2 Receptor alpha in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Unpurified ab128955 showing positive staining in Hodgkin's lymphoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Unpurified ab128955 showing negative staining in Human liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Unpurified ab128955 showing positive staining in Human thymus tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

  • Unpurified ab128955 showing positive staining in Human spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

References

ab215378 has not yet been referenced specifically in any publications.

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