Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)

Mouse monoclonal IL-2 Receptor alpha antibody [MEM-181]. Validated in Flow Cyt, ICC/IF and tested in Mouse, Human. Cited in 2 publication(s). Independently reviewed in 1 review(s).

Reviews (1)Q&A (5)References (3)

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [MEM-181]
    See all IL-2 Receptor alpha primary antibodies

  • Description

    Mouse monoclonal [MEM-181] to IL-2 Receptor alpha

  • Host species

    Mouse

  • Specificity

    CD25 antigen (human).

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Tissue, cells or virus corresponding to Human IL-2 Receptor alpha.

  • Epitope

    extracellular

Properties

Applications

Our Abpromise guarantee covers the use of ab8235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/1000.

Target

  • Function

    Receptor for interleukin-2.

  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2 Receptor alpha antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    Images

    • Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)
      Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)
      Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References

    This product has been referenced in:

    • Zhang Q  et al. Interaction of transforming growth factor-ß-Smads/microRNA-362-3p/CD82 mediated by M2 macrophages promotes the process of epithelial-mesenchymal transition in hepatocellular carcinoma cells. Cancer Sci 110:2507-2519 (2019). Read more (PubMed: 31215741) »
    • Brusés JL Cell surface localization of a3ß4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS One 8:e62435 (2013). ICC/IF ; Human . Read more (PubMed: 23626818) »
    See all 3 Publications for this product

    Publishing research using ab8235? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question

    https://www.abcam.com/IL2-Receptor-alpha-antibody-MEM-181-ab8235.html PLEASE IS THE ANIBODY NEED SCOND ANTIBODY OR NO  

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    Answer

    The antibody ab8232 is unconjugated and so you would need to use a secondary antibody for detection. If you tell me what techniques you are using I would be happy to make some suggestions about suitable secondary antibodies. Please let me know if there is anything else I can help you with.

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-2 Receptor alpha antibody [MEM-181]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293 tranfected with chimeric receptor construct)
    Specification
    HEK293 tranfected with chimeric receptor construct
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 17 2008

    Question

    I am working on a IL2R construct. I will transfect cells, and then use the antibody ab8235 for immunocytochemistry. the IL2R protein is supposed to be localized to the plasma membrane. I went over abreview of this product, and I realized that the lab that has used it for ICC, had permealized the cells with 0.5% Triton X-100 or 0.5% Saponin. My question is do I really need the permeabilisation. As the IL2R chimeric construct is supposed to be present on the cell membrane, then I would expect the antibody to bind to the protein without permeabilisation!! I actually need to to skip the permeabilisation step, because I want to try the used the anitbody for construct clustering. another question that might help me get an answer, is whether the epitope is extracellular or not. thanks

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    Answer

    I can confirm that this product does not required pemeabilisation when it was tested in FACS as the recognized epitope is extracellular. Please do not hesitate to contact us if you require further information.

    Read More

    Question

    I am looking for an antibody that will specifically bind to the IL-2Receptor alpha subunit and will also block IL-2 binding. I cannot find any information about this for ab8235. Can you confirm whether this antibody blocks IL-2 binding, and if not, could you recommend any antibodies for me?

    Read More
    Answer

    Thank you for your enquiry. I ran a search for a neutralising antibody directed against the IL2 Receptor alpha and unfortunately could find any results. This does not mean that the ab8235 will not work in blocking IL2, it just means that we have not tested any of the IL2 Receptor alpha in neutralisation. If you do decide to purchase the antibody for blocking, we can not guarantee that it will work, but please let us know how it goes! You can earn abpoints towards many promotions by filling out Abreviews about your experience. Also, at the bottom of any search results page, there is a link to the World Antibody Gateway which can search 249 online antibody companies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    We have used the antibody but have not been successful in Western blotting. It is likely that the antibody is conformation senstive and the rigours of SDS and mercaptoathanol have rendered it non-recognisable.

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    Answer

    Thank you for your message. We are very sorry but there was a discrepancy on our datasheet. We would like to confirm that this antibody has not been tested and characterised for Western blot application. Thank you for your feedback; it is very likely that the antibody recognizes only the native form of the protein and it is not suitable for WB. We can offer you either a credit note or a refund. Would you be so kind to let us know how you would prefer to proceed? Please could you confirm your Purchase Order Number or your Abcam Order Number. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

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    Question

    I have just purchased the above antibody as your catalogue stated that it was suitable for Western blot. The data sheet provided with the antibody does not state that it has been tested with WB, and doesn't provide any working ranges.please could you clarify the utility of this antibody under these conditions? many thanks

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    Answer

    Our datasheet currently states that the antibody has not yet been tested in WB and I apologise if it previously did; I have checked with our source and they confirmed they have not tested it yet. Should you wish a refund can you please give me your order number and/or Abcam order number and I will arrange this straight away. If you still wish to test ab8235 I would recommend a preliminary experiment to test which dilution would be best: try 1:200, 1:500, 1:1000 and 1:2000 and optimise the dilution according to your results. Please let us know how you get on with ab8235 in WB and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher. Please do not hesitate to contact me if you need further advice,

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