Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)
![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)](https://www.abcam.com/ps/products/8/ab8235/Images/ab8235-13995-anti-il-2-receptor-alpha-antibody-mem-181-flow-cytometry.jpg "Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)")
Key features and details
- Mouse monoclonal [MEM-181] to IL-2 Receptor alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [MEM-181]
See all IL-2 Receptor alpha primary antibodies -
Description
Mouse monoclonal [MEM-181] to IL-2 Receptor alpha -
Host species
Mouse -
Specificity
CD25 antigen (human). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human IL-2 Receptor alpha. PHA-activated peripheral blood leucocytes
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Epitope
extracellular -
Positive control
- Flow Cyt: Jurkat cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-181 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-IL-2 Receptor alpha antibody [MEM-181], prediluted (ab157308)
- FITC Anti-IL-2 Receptor alpha antibody [MEM-181] (ab18275)
- PE/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181] (ab234252)
- Biotin Anti-IL-2 Receptor alpha antibody [MEM-181] (ab239240)
- APC/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181] (ab239307)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab8235 has been referenced in 4 publications.
- Zhou R et al. Combined effects of BPA and PFOS on fetal cardiac development: In vitro and in vivo experiments. Environ Toxicol Pharmacol 80:103434 (2020). PubMed: 32544427
- Zhang Q et al. Interaction of transforming growth factor-ß-Smads/microRNA-362-3p/CD82 mediated by M2 macrophages promotes the process of epithelial-mesenchymal transition in hepatocellular carcinoma cells. Cancer Sci 110:2507-2519 (2019). PubMed: 31215741
- Brusés JL Cell surface localization of a3ß4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS One 8:e62435 (2013). ICC/IF ; Human . PubMed: 23626818
- Nielsen MS et al. Different motifs regulate trafficking of SorCS1 isoforms. Traffic 9:980-94 (2008). ICC/IF ; Mouse . PubMed: 18315530