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  1. Link

    il-2-receptor-alpha-antibody-mem-181-prediluted-percpcy55-ab157308.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-IL-2 Receptor alpha antibody [MEM-181], prediluted (PerCP/Cy5.5®) (ab157308)

  • Datasheet
  • SDS
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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [MEM-181], prediluted (PerCP/Cy5.5®)
    See all IL-2 Receptor alpha primary antibodies
  • Description

    Mouse monoclonal [MEM-181] to IL-2 Receptor alpha, prediluted (PerCP/Cy5.5®)
  • Host species

    Mouse
  • Conjugation

    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human IL-2 Receptor alpha.

  • General notes

    Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Clonality

    Monoclonal
  • Clone number

    MEM-181
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-IL-2 Receptor alpha antibody [MEM-181] (FITC) (ab18275)
    • Anti-IL-2 Receptor alpha antibody [MEM-181] (PE/Cy7 ®) (ab234252)
    • Anti-IL-2 Receptor alpha antibody [MEM-181] (Biotin) (ab239240)
    • Anti-IL-2 Receptor alpha antibody [MEM-181] (Allophycocyanin/Cy7 ®) (ab239307)
    • Anti-IL-2 Receptor alpha antibody [MEM-181] (Phycoerythrin) (ab36419)
    • Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)
  • Isotype control

    • Mouse IgG1, Kappa Monoclonal [MOPC-21] (PerCP/Cy5.5®) - Isotype Control (ab157226)
  • Recombinant Protein

    • Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)

Applications

Our Abpromise guarantee covers the use of ab157308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

(or 100 µl of whole blood).

 

 

 

ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Receptor for interleukin-2.
  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P01589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3559 Human
    • Omim: 147730 Human
    • SwissProt: P01589 Human
    • Unigene: 231367 Human
    • Alternative names

      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2 Receptor alpha antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    Protocols

    • Flow cytometry protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Drbal K  et al. Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol 19:675-84 (2007). Read more (PubMed: 17446208) »
    See 1 Publication for this product

    Publishing research using ab157308? Please let us know so that we can cite the reference in this datasheet.

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