Anti-IL-2 Receptor alpha antibody [OX39] - BSA and Azide free (ab244557)
Key features and details
- Mouse monoclonal [OX39] to IL-2 Receptor alpha - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [OX39] - BSA and Azide free
See all IL-2 Receptor alpha primary antibodies -
Description
Mouse monoclonal [OX39] to IL-2 Receptor alpha - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to IL-2 Receptor alpha.
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Positive control
- Flow Cyt: Lewis rat splenocytes treated with 5 g/ml ConA for 3 days.IHC-Fr: Normal rat spleen tissue.
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General notes
ab244557 is the PBS only format of ab6411.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX39 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab244557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | Use a concentration of 0.2 µg/ml. | |
IHC-Fr | Use a concentration of 5 µg/ml. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25704 Rat
- SwissProt: P26897 Rat
- Unigene: 9872 Rat
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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This data was developed using the same antibody clone in a different buffer formulation (ab6411).
Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5 µg/ml ConA for 3 days (bottom) were stained with ab6411 (right) or mouse IgG1 kappa (ab170190) isotype (left).
Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6411) or mouse IgG1 kappa (ab170190) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.
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Immunohistochemistry (Frozen sections) - Anti-IL-2 Receptor alpha antibody [OX39] - BSA and Azide free (ab244557)
This data was developed using the same antibody clone in a different buffer formulation (ab6411).
IHC image of IL2 receptor alpha staining in a section of frozen normal rat spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6411 at 5µg/ml and ab16669 (Rabbit monoclonal [SP7] to CD3) at 1/150. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor®488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244557 has not yet been referenced specifically in any publications.