Key features and details
- Rat monoclonal [PC61.5] to IL-2 Receptor alpha (FITC)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-IL-2 Receptor alpha antibody [PC61.5] (FITC)
See all IL-2 Receptor alpha primary antibodies
DescriptionRat monoclonal [PC61.5] to IL-2 Receptor alpha (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Mouse C57Bl/6 spleen cells.
ab210332 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25-. This clone has also been reported for depletion of Treg cells in vivo.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
PurityProtein A purified
Purification notesab210332 was purified from tissue culture supernatant and conjugated under optimal conditions, with unreacted dye removed from the preparation.
Light chain typelambda
- Anti-IL-2 Receptor alpha antibody [PC61.5] (APC) (ab210330)
- Anti-IL-2 Receptor alpha antibody [PC61.5] - Low endotoxin, Azide free (ab210333)
- Anti-IL-2 Receptor alpha antibody [PC61.5] (PE) (ab210334)
- Anti-IL-2 Receptor alpha antibody [PC61.5] (PE/Cy7®) (ab210335)
- Anti-IL-2 Receptor alpha antibody [PC61.5] (PerCP/Cy5.5®) (ab210336)
Our Abpromise guarantee covers the use of ab210332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use at 0.125 µg.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesContains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
Flow cytometric analysis of mouse C57Bl/6 spleen cells stained with APC Anti-Mouse IL-2 Receptor alpha antibody and ab210332 at 0.125 µg (right panel) or FITC Rat IgG1 isotype control at 0.125 µg (left panel).
ab210332 has not yet been referenced specifically in any publications.