Anti-IL-2 Receptor alpha antibody [PC61.5] - Low endotoxin, Azide free (ab210333)
Key features and details
- Rat monoclonal [PC61.5] to IL-2 Receptor alpha - Low endotoxin, Azide free
- Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [PC61.5] - Low endotoxin, Azide free
See all IL-2 Receptor alpha primary antibodies -
Description
Rat monoclonal [PC61.5] to IL-2 Receptor alpha - Low endotoxin, Azide free -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
Endotoxin Level: Less than or equal to 0.01 EU/µg, as determined by the LaL assay.
ab210333 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purity >90% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
PC61.5 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16184 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab210333 has been referenced in 1 publication.
- Sami E et al. The Immunosuppressive Microenvironment in BRCA1-IRIS-Overexpressing TNBC Tumors Is Induced by Bidirectional Interaction with Tumor-Associated Macrophages. Cancer Res 80:1102-1117 (2020). PubMed: 31911557