Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - PBS with 0.025% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 1% BSA
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary A
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Abcam has not tested ab231441 in Rat samples or in Frozen tissue samples and we do not know how the antibody should perform in these samples. This customer received a credit for testing the antibody in an untested experiment as part of our Abtrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Neetu Kushwah
Verified customer
Submitted Jan 05 2022