Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-21
  • Host species

    Rabbit
  • Specificity

    Peptide sequence is < 50 % identical to other interleukins in this region and is therefore not expected to cross-react.
  • Tested applications

    Suitable for: ELISA, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human IL-21 aa 118-146 (C terminal).
    Sequence:

    CDSYEKKPPKEFLERFKSLLQKMIHQHLS

  • Positive control

    • Human spleen cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 0.21% Potassium phosphate, 0.812% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
ICC 1/500.

Target

  • Function

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • Tissue specificity

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • Sequence similarities

    Belongs to the IL-15/IL-21 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CVID11 antibody
    • IL 21 antibody
    • IL-21 antibody
    • Il21 antibody
    • IL21_HUMAN antibody
    • Interleukin 21 antibody
    • Interleukin-21 antibody
    • interleukin-21 isoform antibody
    • Interleukin21 antibody
    • OTTHUMP00000164088 antibody
    • Za11 antibody
    see all

References

This product has been referenced in:

  • Longtine MS  et al. Calcitriol regulates immune genes CD14 and CD180 to modulate LPS responses in human trophoblasts. Reproduction 154:735-744 (2017). Read more (PubMed: 29089453) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your email. These two antibodies have not been tested together in a sandwich ELISA and sensitivity information is not available. However, both do detect different regions of human IL21. We do have a general sandwich ELISA protocol which you can refer to; it is located in the protocols tab on the online datasheet. Please contact us again if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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