Overview

  • Product name
  • Description
    Goat polyclonal to IL-22
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human IL-22 aa 148-177 (C terminal).
    Sequence:

    LKDTVKKLGESGEIKAIGELDLLFMSLRNA

  • Positive control
    • Human spleen and tonsil.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 0.21% Potassium phosphate, 0.812% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
ICC 1/300.
IHC-P Use at an assay dependent dilution. PubMed: 21182091

Target

  • Function
    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytokine Zcyto18 antibody
    • IL 10 related T cell derived inducible factor antibody
    • IL 21 antibody
    • IL 22 antibody
    • IL D110 antibody
    • IL TIF antibody
    • IL-10-related T-cell-derived-inducible factor antibody
    • IL-22 antibody
    • IL-TIF antibody
    • IL21 antibody
    • Il22 antibody
    • IL22_HUMAN antibody
    • ILD110 antibody
    • ILTIF antibody
    • Interleukin 10 related T cell derived inducible factor antibody
    • interleukin 21 antibody
    • Interleukin 22 antibody
    • Interleukin-22 antibody
    • MGC79382 antibody
    • MGC79384 antibody
    • TIFa antibody
    • TIFIL 23 antibody
    • TIFIL23 antibody
    • UNQ3099/PRO10096 antibody
    • zcyto18 antibody
    see all

Images

  • ab18498 (1/300) used for the immunohistochemical detection of human C-terminal IL22 on a paraffin embedded section of human tonsil.

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Inflammed Colon, Normal Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Inflammed Colon, Normal Spleen
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2015

Answer

Thank you for contacting Abcam.

I can see no reason why you cannot use a fluorescently tagged secondary antibody with these primariesin your IHC-P experiments (for ab5690, there is even an IHC-Fr image, where they used a fluorescent secondary for their detection).

Below are links to some secondary’s that could be used with these primaries.

For ab18498: https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-H-L-DyLight-488-pre-adsorbed-ab96915.html

For ab5690: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab96897.html

For ab91494, since we do not have any anti-Hamster fluorescent secondary’s in our catalogue, you could use one of our EasyLink conjugation kits, which allows you to directly label the primary with a fluorescent tag, please see the link below for more details:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink



Please let me know if there is anything else I can help you with.

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Question
Answer

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab18498 in horse, WB and flow cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for horse, WB and/or flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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