Anti-IL-22 antibody [IMG8F11E2] (ab134035)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22 antibody [IMG8F11E2] (ab134035)
  • Western blot - Anti-IL-22 antibody [IMG8F11E2] (ab134035)

Key features and details

  • Mouse monoclonal [IMG8F11E2] to IL-22
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-IL-22 antibody [IMG8F11E2]
    See all IL-22 primary antibodies

  • Description

    Mouse monoclonal [IMG8F11E2] to IL-22

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human IL-22.

  • Positive control

    • Recombinant Human IL22; Human spleen cell lysate; Human brain and kidney tissues.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.

  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    IMG8F11E2

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P (1)
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    Cytokine that contributes to the inflammatory response in vivo.

  • Sequence similarities

    Belongs to the IL-10 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cytokine Zcyto18 antibody
      • IL 10 related T cell derived inducible factor antibody
      • IL 21 antibody
      • IL 22 antibody
      • IL D110 antibody
      • IL TIF antibody
      • IL-10-related T-cell-derived-inducible factor antibody
      • IL-22 antibody
      • IL-TIF antibody
      • IL21 antibody
      • Il22 antibody
      • IL22_HUMAN antibody
      • ILD110 antibody
      • ILTIF antibody
      • Interleukin 10 related T cell derived inducible factor antibody
      • interleukin 21 antibody
      • Interleukin 22 antibody
      • Interleukin-22 antibody
      • MGC79382 antibody
      • MGC79384 antibody
      • TIFa antibody
      • TIFIL 23 antibody
      • TIFIL23 antibody
      • UNQ3099/PRO10096 antibody
      • zcyto18 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22 antibody [IMG8F11E2] (ab134035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22 antibody [IMG8F11E2] (ab134035)
      Immunohistochemical analysis of Paraffin-embedded Human brain tissue (top) and Human kidney tissue (bottom) labelling IL22 with ab134035 at 5µg/ml.
    • Western blot - Anti-IL-22 antibody [IMG8F11E2] (ab134035)
      Western blot - Anti-IL-22 antibody [IMG8F11E2] (ab134035)
      All lanes : Anti-IL-22 antibody [IMG8F11E2] (ab134035) at 1 µg/ml

      Lane 1 : Recombinant Human IL22 protein
      Lane 2 : Human spleen cell lysate

      Secondary
      All lanes : Goat anti-mouse HRP

      Developed using the ECL technique.

      Predicted band size: 20 kDa

    References (1)

    Publishing research using ab134035? Please let us know so that we can cite the reference in this datasheet.

    ab134035 has been referenced in 1 publication.

    • Orfali RL  et al. Staphylococcus aureus enterotoxins modulate IL-22-secreting cells in adults with atopic dermatitis. Sci Rep 8:6665 (2018). PubMed: 29703987

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL22 antibody [IMG8F11E2]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Permeabilization
    No
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    Submitted Jan 26 2015

    Question

    Dear Mrs., Dear Mr.,

    Could you please send me publication references for ICH-P of the following product: ab134035 (Anti-IL22 antibody [IMG8F11E2]).

    Sincerely yours,

    Read More
    Answer

    Thank you for your enquiry.
    I am pleased to provide the following list of publications which have used ab134035 IL22 antibody [IMG8F11E2]

    1. Zenewicz, LA and RA Flavell 2011 Recent Advances in IL-22 Biology Intl Immunol. 23: 159.

    2. Lejeune, D., et al 2002. J. Biol. Chem. 277:33676.

    3. Duhen, T., et al 2009. Nat. Immunol. 10:857.

    4. Trifari, S. et al 2009. Nat. Immunol. 10:864.

    5. Veldhoen, et al. 2008. Nature 453:106.

    6. Pickert, G., et al. 2009. J. Exp. Med. 206:1465.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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