Overview

  • Product name
  • Description
    Goat polyclonal to IL-22RA1
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, ICC, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-22RA1 aa 42-70 (N terminal).
    Sequence:

    DSGPEGTPDTVYSIEYKTYGERDWVAKK

  • Positive control
    • Human spleen or tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab18568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
IHC-Fr
IHC-P
  • Application notes
    ELISA: 1/100000.
    IHC-P: 1/100. Perform heat mediated antigen retrieval via the pressure cooker method (with citrate buffer pH 6 or pH 8) before commencing with IHC staining protocol. See recommended protocol.
    IHC-Fr: Use at an assay dependent dilution. Fix with ice cold acetone. See recommended protocol.
    ICC (cytospun cells on slides): Use at an assay dependent dilution. Fix with ice cold acetone. See recommended protocol.


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • CRF2 9 antibody
      • CRF2-9 antibody
      • Cytokine receptor class-II member 9 antibody
      • cytokine receptor classII member 9 antibody
      • Cytokine receptor family 2 member 9 antibody
      • I22R1_HUMAN antibody
      • IL-22 receptor subunit alpha-1 antibody
      • IL-22R-alpha-1 antibody
      • IL-22RA1 antibody
      • IL22 Receptor Alpha antibody
      • IL22R antibody
      • IL22R1 antibody
      • Il22ra1 antibody
      • ILTIF R1 chain antibody
      • Interleukin 22 Receptor alpha 1 antibody
      • Interleukin 22 Receptor antibody
      • Interleukin-22 receptor subunit alpha-1 antibody
      • ZcytoR11 antibody
      see all

    Images

    • ab18568 employed for the immunohistochemical detection of human N-terminal IL-22RA1, in paraffin embedded human spleen.

    • ab18568 employed for the immunohistochemical detection of human N-terminal IL-22RA1, in paraffin embedded human tonsil.

    References

    ab18568 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for e-mail and updating me on your experiments with ab18568, I would recommend trying with tritonx100 in the dilution buffer as in my experience this can help a lot the penetration of the antibodies into the tissue. We have recently added images of the staining of human tonsil and human spleen with a18568, and you can access those by clicking on the link to this datasheet below. Regarding the isotype control, unfortunately I was unable to find a goat one in our catalogue, I would therefore suggest simply omitting the primary antibody as a secondary control. I hope these information will help, if you still experience problems please let me know and I can offer you a replacement or refund if you purchased the antibody in the last 90days,

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    Answer

    Thank you for your questions, I am unable to find out if there is a major difference between the different Dako products and would suggest calling Dako and asking their technical support what the difference is, especially the pH or concentration of citrate, sorry I can't help more. I have double checked with our IHC-paraffin specialist and he confirmed that the microwave steaming in 99% of cases does the same type of antigen retrieval than a steamer, can I please make sure you do not use a domestic microwave as this gives hot and cold spots which can damage the antigen a lot? If you still experience problems you may unfortunatly have to consider a steamer. I hope the above advice will help you, please let me know if I can be of further assistance,

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    Answer

    Thank you for contacting us for some technical support with this anti IL22 receptor antibody, I am trying to find out what antigen retrieval solution was used to test this antibody in formalin fixed paraffin embedded sections as it is possible that the problem you are experiencing is due to an unsuitable antigen retrieval step. Could you please confirm that you incubated the tissue sections in antibody diluted 1:300 or lower (trying also 1:100 could potentially help) overnight at 4C and that the antibody (primary and secondary) dilution buffer contained tritonx100 (0.3%v/v)? If you have not used this permeabilising agent in the dilution buffers I would recommend trying this as this may help penetration of the antibody into the tissue sections. I'm also wondering if the problem could be due to the secondary antibody? It would be useful to test the secondary with other primary antibodies to make sure it is not damaged, and can I please confirm that the secondary was incubated 1-2hrs at RT? I'm sorry for the delay in finding out the recommended antigen retrieval method, as soon as I find out I will let you know, your patience is appreciated and I hope the above suggestion will help you,

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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