Recombinant Anti-IL-22RA1 antibody [EPR24559-15] - BSA and Azide free (ab283701)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24559-15] to IL-22RA1 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-22RA1 antibody [EPR24559-15] - BSA and Azide free
See all IL-22RA1 primary antibodies -
Description
Rabbit monoclonal [EPR24559-15] to IL-22RA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon, Human colon cancer, SW480 cell line.
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General notes
ab283701 is the carrier free version of ab283677.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24559-15 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 58985 Human
- Omim: 605457 Human
- SwissProt: Q8N6P7 Human
- Unigene: 110915 Human
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Alternative names
- CRF2 9 antibody
- CRF2-9 antibody
- Cytokine receptor class-II member 9 antibody
see all
Images
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This data was developed using ab283677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-22RA1 with ab283677 at 1/500 (1.178 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID:29742509). The section was incubated with ab283677 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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This data was developed using ab283677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labelling IL-22RA1 with ab283677 at 1/500 (1.178 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon cancer (PMID:29742509). The section was incubated with ab283677 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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This data was developed using ab283677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded SW480 (Human colorectal adenocarcinoma epithelial cell) tissue labelling IL-22RA1 with ab283677 at 1/100 (1.178 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on SW480 cells. The section was incubated with ab283677 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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This data was developed using ab283677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Jurkat (Human T cell leukemia T lymphocyte) labelling IL-22RA1 with ab283677 at 1/100 (1.178 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was incubated with ab283677 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Negative control: No staining on Jurkat cells (PMID:15308104).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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This data was developed using ab283677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded THP-1 ( Human monocytic leukemia monocyte) labelling IL-22RA1 with ab283677 at 1/100 (1.178 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was incubated with ab283677 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Negative control: No staining on THP-1 cells (PMID:15308104).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab283701 has not yet been referenced specifically in any publications.