Anti-IL-23 antibody (ab45420)
Key features and details
- Rabbit polyclonal to IL-23
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-23 antibody
See all IL-23 primary antibodies -
Description
Rabbit polyclonal to IL-23 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-23 (C terminal). 14 amino acid peptide located within the last 50 amino acids of IL23.
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Positive control
- Raji cells and Raji cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 10 µg/ml.
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
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| Notes |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Tissue specificity
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
Sequence similarities
Belongs to the IL-6 superfamily. -
Developmental stage
Expressed by newborns dendritic cells. -
Cellular localization
Secreted. Secreted upon association with IL12B. - Information by UniProt
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Database links
- Entrez Gene: 3593 Human
- Entrez Gene: 51561 Human
- Omim: 161561 Human
- Omim: 605580 Human
- SwissProt: P29460 Human
- SwissProt: Q9NPF7 Human
- Unigene: 382212 Human
- Unigene: 98309 Human
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Alternative names
- IL 23 A antibody
- IL 23 antibody
- IL 23 subunit alpha antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody (ab45420)
Immunohistochemistry of IL-23 in human pancreas tissue with ab45420 at 5 µg/mL.
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Western blot - Anti-IL-23 antibody (ab45420)All lanes : Anti-IL-23 antibody (ab45420) at 1 µg/ml
Lane 1 : Raji lysate
Lane 2 : Raji lysate with IL23 P19 blocking peptide
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-IL23 antibody (ab45420)This image is courtesy of an anonymous AbreviewAb45420 staining IL23 in Mouse bone marrow derived macrophages by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody at 1/200 dilution in 5% goat serum in PBS for 1 hour at 20°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)ab45420 at 10 µg/ml staining IL23 in Raji cells by ICC/IF -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)This image is courtesy of an Abreview submitted by Dr James Harrisab45420 staining IL23 P19 (green) in Human THP-1 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in 5% goat serum + 1 BSA) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)Immunofluorescence of IL-23 in Raji cells using ab45420 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab45420 has been referenced in 21 publications.
- Montanaro M et al. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability. Int J Mol Sci 22:N/A (2021). PubMed: 33401449
- Hou C et al. Resveratrol reduces drug resistance of SCLC cells by suppressing the inflammatory microenvironment and the STAT3/VEGF pathway. FEBS Open Bio N/A:N/A (2021). PubMed: 34129726
- Du HL et al. Synergistic effect of halofuginone and dexamethasone on LPS-induced acute lung injury in type II alveolar epithelial cells and a rat model. Mol Med Rep 21:927-935 (2020). PubMed: 31974595
- Wang H et al. NLRP3 inflammasome activates interleukin-23/interleukin-17 axis during ischaemia-reperfusion injury in cerebral ischaemia in mice. Life Sci 227:101-113 (2019). PubMed: 31002919
- Nakajima K et al. Stat3 activation in epidermal keratinocytes induces Langerhans cell activation to form an essential circuit for psoriasis via IL-23 production. J Dermatol Sci 93:82-91 (2019). PubMed: 30514663