Anti-IL-23 antibody [HLT2736] (ab110630)
Key features and details
- Mouse monoclonal [HLT2736] to IL-23
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-IL-23 antibody [HLT2736]
See all IL-23 primary antibodies -
Description
Mouse monoclonal [HLT2736] to IL-23 -
Host species
Mouse -
Specificity
Human recombinant p40 and p19 with elastin linker. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human IL-23.
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Positive control
- Human Adrenal and Lung vasculature tissues; Recombinant Human IL23.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HLT2736 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Tissue specificity
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
Sequence similarities
Belongs to the IL-6 superfamily. -
Developmental stage
Expressed by newborns dendritic cells. -
Cellular localization
Secreted. Secreted upon association with IL12B. - Information by UniProt
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Database links
- Entrez Gene: 3593 Human
- Entrez Gene: 51561 Human
- Omim: 161561 Human
- Omim: 605580 Human
- SwissProt: P29460 Human
- SwissProt: Q9NPF7 Human
- Unigene: 382212 Human
- Unigene: 98309 Human
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Alternative names
- IL 23 A antibody
- IL 23 antibody
- IL 23 subunit alpha antibody
see all
Images
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Immunohistological staining of Human Adrenal tissue, Formalin-Fixed, Paraffin-Embedded using ab110630 at 10µg/ml dilution.
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Immunohistological staining of Human Lung vasculature tissue, Formalin-Fixed, Paraffin-Embedded using ab110630 at 10µg/ml dilution.
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Anti-IL-23 antibody [HLT2736] (ab110630) + Recombinant Human IL23 P19
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab110630 has been referenced in 2 publications.
- Folkmanis K et al. Clinicopathological Significance of Exosomal Proteins CD9 and CD63 and DNA Mismatch Repair Proteins in Prostate Adenocarcinoma and Benign Hyperplasia. Diagnostics (Basel) 12:N/A (2022). PubMed: 35204378
- Helbling M et al. Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients. Oncotarget 5:4671-82 (2014). IHC . PubMed: 25015728