Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human IL-25 aa 33-177.
    Database link: BC069565

  • Positive control

    • Human fetal lung and kidney lysates; Human fetal prostate tissue.
  • General notes

     This product was previously labelled as IL-17E

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Buffer contains non-immune IgG.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  • Tissue specificity

    Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IL 17E antibody
    • IL 25 antibody
    • IL-17E antibody
    • IL-25 antibody
    • IL17e antibody
    • IL25 antibody
    • IL25_HUMAN antibody
    • Interleukin 17E antibody
    • Interleukin 25 antibody
    • Interleukin-17E antibody
    • Interleukin-25 antibody
    • Interleukin17E antibody
    • Interleukin25 antibody
    • OTTHUMP00000027946 antibody
    • OTTHUMP00000246238 antibody
    • UNQ3120/PRO10272 antibody
    see all

Images

  • All lanes : Anti-IL-25 antibody (ab108530) at 1/500 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human fetal kidney lysate

    Predicted band size: 20 kDa

  • ab108530 at 1/100 dilution staining IL17E in Human fetal prostate by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References

This product has been referenced in:

  • Xu J  et al. Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps. PLoS One 11:e0161013 (2016). ICC/IF . Read more (PubMed: 27584662) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us yesterday.

As discussed over the phone I have now looked into which antibodies we have against IL17E which would be likely to cross react with mouse samples. All the antibodies we have against this target are raised against immunogens taken from the human protein.

However, we have a couple of antibodies where the immunogen used to raise the antibody shares high homology with the mouse protein and would therefore be predicted to react. As discussed over the phone, the immunogen used to raise the antibody ab108530 shares only 71% homology and we would therefore not recommend trying this. I have removed the reference to "predicted to react" with mouse for this antibody to avoid future confusion.

I have found that the mouse monoclonal antibody ab13651 has actually already been tested with mouse testis samples and found to detect a band between 14-21 kDa in Western blotting. I would therefore be able to offer the Abpromise for this antibody. This would mean that if you purchased this antibody and you were not able to get good results with mouse samples you would be eligible for a replacement antibody of your choice, a credit note or refund.

I understand you would prefer an antibody raised in rabbit and we do have one that may be worth trying. The rabbit polyclonal ab123666 has been raised against the amino acid residues 74-104 of human IL17E which matches exactly to the mouse protein (SwissProt reference Q8VHH8) apart from 3 residues. We would therefore expect the antibody to be able to cross react. If you would like to participate in the testing discount scheme with this antibody I can raise the discount code for you.

As discussed over the phone, this offer means that if you purchase ab123666 as normal, test the antibody with your mouse samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab123666 off any product). More information on this offer can be found from the following link:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab123666.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your phone call.

Thelabhas confirmed that the buffer for antibody ab108530 contains non-immune IgG as a stabilizer. I sincerely apologize for this not being mentioned on our datasheet. I have asked my colleague to update our datasheet to reflect this information. I would be more than happy to send you a free of charge replacement antibody or offer a credit or refund. Please let me know your original order or PO number and how you would like to proceed and I will be happy to assist you further.

For ab17613, my colleague has confirmed that it is sterile filtered and lyophilized from PBS, pH 7.2 and is recommended to be reconstituted with sterile water to a concentration of 0.1-1.0mg/mL. Regarding the unsuccessful conjugation, she recommended checking the pH of the reconstituted antibody to ensure that it is within the tolerance of the kit. She also suggested optimizing the amount of dye to ensure the appropriate molar ratio.

I hope this helps, please let me know if you need any additional information or assistance.

Read More

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