Product nameAnti-IL-25 antibody (Biotin)
See all IL-25 primary antibodies
DescriptionRabbit polyclonal to IL-25 (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- WB: Recombinant human IL-25.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271280 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-25 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionInduces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Tissue specificityExpressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Sequence similaritiesBelongs to the IL-17 family.
- Information by UniProt
- IL 17E antibody
- IL 25 antibody
- IL-17E antibody
Lane 1: Marker.
Lane 2: Recombinant hIL-25 at 250ng/lane.
Lane 3: Recombinant hIL-25 at 125ng/lane.
Lane 4: Recombinant hIL-25 at 62.5ng/lane.
Lane 5: Recombinant hIL-25 at 31.25ng/lane.
Lane 6: Recombinant hIL-25 at 15.625ng/lane.
Lane 7: Recombinant hIL-25 at 7.8ng/lane.
Lane 8: Recombinant hIL-25 at 3.9ng/lane.
Lane 9: Recombinant hIL-25 at 1.95ng/lane.
Lane 10: Recombinant hIL-25 at 0.975ng/lane.
Lane 11: Recombinant hIL-25 at 0.4875ng/lane.
Lane 12: Recombinant hIL-25 at 0.24ng/lane.
Human IL-17E (IL-25) was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271280. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-17E protein.
ab271280 has not yet been referenced specifically in any publications.