Recombinant Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free (ab250239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5743(2)] to IL-25 - BSA and Azide free
- Suitable for: WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free
See all IL-25 primary antibodies -
Description
Rabbit monoclonal [EPR5743(2)] to IL-25 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250239 is the carrier-free version of ab180594.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5743(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 20 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. -
Tissue specificity
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 64806 Human
- Omim: 605658 Human
- SwissProt: Q9H293 Human
- Unigene: 302036 Human
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Alternative names
- IL 17E antibody
- IL 25 antibody
- IL-17E antibody
see all
Images
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Anti-IL-25 antibody [EPR5743(2)] (ab180594) at 1/5000 dilution + Human IL-17E recombinant protein aa1-145 at 0.05 µg
Secondary
Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?This data was developed using ab180594, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250239 has not yet been referenced specifically in any publications.