Key features and details
- Mouse monoclonal [6H9E6] to IL-28A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IL-28A antibody [6H9E6]
See all IL-28A primary antibodies
DescriptionMouse monoclonal [6H9E6] to IL-28A
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-28A aa 1-200. (Expressed in E.coli).
MKLDMTGDCTPVLVLMAAVLTVTGAVPVARLHGALPDARGCHIAQFKSLS PQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEA ELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPR TRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV
Database link: Q8IZJ0
- WB: Human IL28A (aa 1-200) recombinant protein; IL28A (aa 1-200)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionCytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Sequence similaritiesBelongs to the IL-28/IL-29 family.
- Information by UniProt
- Cytokine ZCYTO20 antibody
- IFN-lambda-2 antibody
- IFNL2 antibody
Anti-IL-28A antibody [6H9E6] (ab233754) at 1/500 dilution + Human IL28A (aa 1-200) recombinant protein
Predicted band size: 22 kDa
Expected MW is 48.2 kDa.
All lanes : Anti-IL-28A antibody [6H9E6] (ab233754) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : IL28A (aa 1-200)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 22 kDa
HeLa (human prostate adenocarcinoma cell line) cells stained for IL28A (green) using ab233754 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233754 has not yet been referenced specifically in any publications.