Anti-IL-28B antibody (ab181411)
Key features and details
- Rabbit polyclonal to IL-28B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-28B antibody
See all IL-28B primary antibodies -
Description
Rabbit polyclonal to IL-28B -
Host species
Rabbit -
Specificity
ab181411 may cross react with IL28A and IL29. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-28B (internal sequence). Peptide corresponds to 17 amino acids near the center. (NP_742151)
Database link: Q8IZI9 -
Positive control
- HeLa cells and cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 22 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. -
Sequence similarities
Belongs to the IL-28/IL-29 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282617 Human
- Omim: 607402 Human
- SwissProt: Q8IZI9 Human
- Unigene: 406744 Human
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Alternative names
- Cytokine ZCYTO22 antibody
- IFN-lambda-3 antibody
- IFN-lambda-4 antibody
see all
Images
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Lane 1 : Anti-IL-28B antibody (ab181411) at 1 µg/ml
Lane 2 : Anti-IL-28B antibody (ab181411) at 2 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells, labeling IL28B using ab181411 at 5 μg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab181411 has not yet been referenced specifically in any publications.