Key features and details
- Rabbit polyclonal to IL-28B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-28B antibody
See all IL-28B primary antibodies
DescriptionRabbit polyclonal to IL-28B
Specificityab181411 may cross react with IL28A and IL29.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human IL-28B (internal sequence). Peptide corresponds to 17 amino acids near the center. (NP_742151)
Database link: Q8IZI9
- HeLa cells and cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 22 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Sequence similaritiesBelongs to the IL-28/IL-29 family.
- Information by UniProt
- Cytokine ZCYTO22 antibody
- IFN-lambda-3 antibody
- IFN-lambda-4 antibody
Lane 1 : Anti-IL-28B antibody (ab181411) at 1 µg/ml
Lane 2 : Anti-IL-28B antibody (ab181411) at 2 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells, labeling IL28B using ab181411 at 5 μg/mL.
ab181411 has not yet been referenced specifically in any publications.