FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Tissue specificityActivated T-cells, mast cells, natural killer cells.
Sequence similaritiesBelongs to the IL-3 family.
- Information by UniProt
- Colony stimulating factor multiple antibody
- Hematopoietic growth factor antibody
- IL 3 antibody
Western blot analysis of varying levels of Recombinant murine IL-3 protein using ab271259 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Mouse mIL-3 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271259. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271259 has not yet been referenced specifically in any publications.