Anti-IL-31 antibody (ab37157)

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Overview

  • Product name

    Anti-IL-31 antibody
    See all IL-31 primary antibodies

  • Description

    Rabbit polyclonal to IL-31

  • Host species

    Rabbit

  • Specificity

    ab37157 recognises Interleukin 31 (IL31).

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Mouse IL-31 (N terminal).
    (Peptide available as ab39776)

  • Positive control

    • RAW264.7 cell lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

  • Tissue specificity

    Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Form

      Amino acids 1-23 are a signal peptide.

    • Alternative names

      • IL 31 antibody
      • IL-31 antibody
      • IL31 antibody
      • IL31_HUMAN antibody
      • Interleukin 31 antibody
      • Interleukin-31 antibody
      see all

    Images

    • Western blot - Anti-IL-31 antibody (ab37157)
      Western blot - Anti-IL-31 antibody (ab37157)
      Lane 1 : Anti-IL-31 antibody (ab37157) at 2.5 µg/ml
      Lane 2 : Anti-IL-31 antibody (ab37157) at 5 µg/ml
      Lane 3 : Anti-IL-31 antibody (ab37157) at 10 µg/ml

      All lanes : RAW264.7 cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 18 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-31 antibody (ab37157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-31 antibody (ab37157)

      ab37157 at 10µg/ml staining IL-31 in rat skeletal muscle tissue by IHC 

    References

    This product has been referenced in:

    • Niyonsaba F  et al. Antimicrobial peptides human beta-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine IL-31 by human mast cells. J Immunol 184:3526-34 (2010). Read more (PubMed: 20190140) »
    See 1 Publication for this product

    Publishing research using ab37157? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a question

    Question

    Hi,



    I was searching for IL31 antibody. This product (ab37157) is interesting to me. But I have a question regarding this product.



    In the data sheet of this antibody, there was a picture showing bands in 3 lanes with different concentration of the antibody. How can you do the western blot on one membrane using different concentration of antibody? Is there any new technology or just a mistake?

    Read More
    Answer

    Thank you for contacting us. The image was labelled incorrectly, all lanes were blotted with a concentration of antobody 10 ug/ml. We are updating the datasheet now, thank you for bringing this error to our attention.

    Read More

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