Product nameAnti-IL-32 antibody
See all IL-32 primary antibodies
DescriptionRabbit polyclonal to IL-32
Specificityab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each).
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human IL-32 (C terminal).
(Peptide available as
- Human spleen lysate, mouse EL4 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab37158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human IL32 peptide (ab39778). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|ELISA||Use at an assay dependent concentration. PubMed: 18544573|
FunctionCytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
Tissue specificitySelectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells.
- Information by UniProt
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
Immunohistochemical analysis of IL32 expression in mouse spleen tissue with ab37158 at 2µg/ml
Lane 1 : Anti-IL-32 antibody (ab37158) at 5 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 10 µg/ml
All lanes : Human spleen cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 12,26 kDa why is the actual band size different from the predicted?
Two isoforms of IL32 can be seen
Westen blot data for human spleen cell lysates (left lanes) and mouse EL4 cell lysates (right lanes).
IHC-P of Human spleen tissue with ab37158 at 10 µg/ml
Immunofluorescence of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.
This product has been referenced in:
- Gomes RS et al. IL-32? promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis. Parasit Vectors 10:336 (2017). IHC-P ; Human . Read more (PubMed: 28709468) »
- Bi D et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). Read more (PubMed: 28912850) »