Anti-IL-32 antibody (ab37158)
Key features and details
- Rabbit polyclonal to IL-32
- Suitable for: IHC-P, WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-32 antibody
See all IL-32 primary antibodies -
Description
Rabbit polyclonal to IL-32 -
Host species
Rabbit -
Specificity
ab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each). -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-32 (C terminal).
(Peptide available asab39778) -
Positive control
- WB: Human spleen lysates. IHC-P: Human spleen tissue. ICC/IF: Human spleen cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).
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ICC/IF |
Use a concentration of 20 µg/ml.
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ELISA |
Use at an assay dependent concentration. PubMed: 18544573
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights). |
ICC/IF
Use a concentration of 20 µg/ml. |
ELISA
Use at an assay dependent concentration. PubMed: 18544573 |
Target
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Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. -
Tissue specificity
Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9235 Human
- Omim: 606001 Human
- SwissProt: P24001 Human
- Unigene: 943 Human
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Alternative names
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
see all
Images
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Lane 1 : Anti-IL-32 antibody (ab37158) at 2 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 4 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 26 kDa -
Lane 1 : Anti-IL-32 antibody (ab37158) at 5 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 10 µg/ml
All lanes : Human spleen cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 12,26 kDa why is the actual band size different from the predicted?
Two isoforms of IL32 can be seen -
IHC-P of Human spleen tissue with ab37158 at 10 µg/ml
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Immunocytochemistry of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab37158 has been referenced in 12 publications.
- Zhang J et al. IL-32 exacerbates adenoid hypertrophy via activating NLRP3-mediated cell pyroptosis, which promotes inflammation. Mol Med Rep 23:N/A (2021). PubMed: 33495843
- Liu D et al. Placenta-derived IL-32ß activates neutrophils to promote preeclampsia development. Cell Mol Immunol 18:979-991 (2021). PubMed: 33707686
- Bengts S et al. Altered IL-32 Signaling in Abdominal Aortic Aneurysm. J Vasc Res 57:236-244 (2020). PubMed: 32434199
- Dali-Youcef N et al. Interleukin-32 Contributes to Human Nonalcoholic Fatty Liver Disease and Insulin Resistance. Hepatol Commun 3:1205-1220 (2019). PubMed: 31497742
- Pavlovic M et al. Diverse Expression of IL-32 in Diffuse and Intestinal Types of Gastric Cancer. Gastroenterol Res Pract 2018:6578273 (2018). PubMed: 30402092