Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5067] to IL-32
- Suitable for: WB
- Reacts with: Human
Product nameAnti-IL-32 antibody [EPR5067]
See all IL-32 primary antibodies
DescriptionRabbit monoclonal [EPR5067] to IL-32
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
corresponding to Human IL-32 aa 200 to the C-terminus.
- Human lymph node and tonsil lysates; Human fetal thymus lysate; HT29 and SW480 lysates; HT29 treated with TNF alpha, SW480 treated with INF gamma and 293T treated with TNF alpha and IFN gamma lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 27 kDa.|
FunctionCytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
Tissue specificitySelectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells.
- Information by UniProt
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
All lanes : Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human fetal thymus lysate
Lane 4 : HT29 treated with TNF alpha lysate
Lane 5 : HT29 lysate
Lane 6 : SW480 treated with INF gamma lysate
Lane 7 : SW480 lysate
Lane 8 : 293T treated with TNF alpha and IFN gamma lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
ab169781 has been referenced in 1 publication.
- Dos Santos JC et al. Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species. PLoS Negl Trop Dis 11:e0005413 (2017). PubMed: 28241012