Recombinant Anti-IL-32 antibody [EPR5067] - BSA and Azide free (ab249525)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5067] to IL-32 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-IL-32 antibody [EPR5067] - BSA and Azide free
See all IL-32 primary antibodies -
Description
Rabbit monoclonal [EPR5067] to IL-32 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human tonsil lysate
-
General notes
ab249525 is the carrier-free version of ab169781.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5067 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
Target
-
Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. -
Tissue specificity
Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 9235 Human
- Omim: 606001 Human
- SwissProt: P24001 Human
- Unigene: 943 Human
-
Alternative names
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
see all
Images
-
Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution + Human tonsil lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 10 secondsThis data was developed using ab169781, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Loading Control: GAPDH
This antibody could detect different isoforms of IL-32.
-
All lanes : Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human fetal thymus lysate
Lane 4 : HT29 treated with TNF alpha lysate
Lane 5 : HT29 lysate
Lane 6 : SW480 treated with INF gamma lysate
Lane 7 : SW480 lysate
Lane 8 : 293T treated with TNF alpha and IFN gamma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDaThis data was developed using ab169781, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab249525 has not yet been referenced specifically in any publications.