Key features and details
- Rabbit polyclonal to IL-33
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-33 antibody
See all IL-33 primary antibodies
DescriptionRabbit polyclonal to IL-33
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human IL-33 (internal sequence) conjugated to keyhole limpet haemocyanin.
- Human lymph node lysate and uterus tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab118503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
modificationsThe full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
- Information by UniProt
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Immunohistochemical staining of paraffin-embedded, formalin fixed, human tonsil lymphocytes with ab118503 at 5 µg/mL.
Negative control - immunohistochemical staining of paraffin-embedded, formalin fixed, human seminiferous tubules with ab118503 at 5 µg/mL.
Human Uterus, Vessels (formalin-fixed, paraffin-embedded) stained with IL33 antibody ab118503 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Lane 1 : Anti-IL-33 antibody (ab118503) at 1 µg/ml
Lane 2 : Anti-IL-33 antibody (ab118503) at 2 µg/ml
All lanes : Human lymph node tissue lysate
Predicted band size: 31 kDa
ab118503 has been referenced in 3 publications.
- Sun Z et al. Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease. J Cell Mol Med N/A:N/A (2018). PubMed: 30478965
- Chen M et al. Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction. Evid Based Complement Alternat Med 2017:2489842 (2017). PubMed: 28751921
- Sun L et al. Serum amyloid A induces interleukin-33 expression through an IRF7-dependent pathway. Eur J Immunol 44:2153-64 (2014). PubMed: 24777946