Anti-IL-33 antibody [Nessy-1] (ab54385)

Mouse monoclonal IL-33 antibody [Nessy-1]. Validated in WB, IP, ELISA, IHC and tested in Mouse, Human. Cited in 7 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name
    Anti-IL-33 antibody [Nessy-1]
    See all IL-33 primary antibodies
  • Description
    Mouse monoclonal [Nessy-1] to IL-33
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human IL-33 aa 112-270.

  • Positive control
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab54385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
  • Tissue specificity
    Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similarities
    Belongs to the IL-1 family. Highly divergent.
  • Domain
    The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • Post-translational
    modifications
    The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
  • Cellular localization
    Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf26 antibody
    • CHROMOSOME 9 OPEN READING FRAME 26 antibody
    • DKFZp586H0523 antibody
    • DVS27 antibody
    • DVS27 related protein antibody
    • IL 1F11 antibody
    • IL 33 antibody
    • IL-1F11 antibody
    • IL-33 antibody
    • IL1F11 antibody
    • IL33 antibody
    • IL33_HUMAN antibody
    • Interleukin 1 family member 11 antibody
    • Interleukin 33 antibody
    • INTERLEUKIN 33 NFHEV antibody
    • Interleukin 33 precursor antibody
    • Interleukin-1 family member 11 antibody
    • Interleukin-33 (109-270) antibody
    • Interleukin33 antibody
    • NF HEV antibody
    • NF-HEV antibody
    • NFEHEV antibody
    • NFHEV antibody
    • Nuclear factor for high endothelial venules antibody
    • Nuclear factor from high endothelial venules antibody
    • OTTHUMP00000021041 antibody
    • RP11 575C20.2 antibody
    see all

Images

  • All lanes : Anti-IL-33 antibody [Nessy-1] (ab54385) at 1/1000 dilution

    Lane 1 : Liver Lysate
    Lane 2 : Liver Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse HRP conjugated polyclonal antibody at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa


    Exposure time: 2 minutes


    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Luo Q  et al. Interleukin-33 Protects Ischemic Brain Injury by Regulating Specific Microglial Activities. Neuroscience 385:75-89 (2018). Read more (PubMed: 29906552) »
  • Yang Y  et al. Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration. Front Pharmacol 9:624 (2018). Read more (PubMed: 29946260) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse RPE/choroid)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris Gel)
Loading amount
25 µg
Treatment
cigarette smoking 6-mo
Specification
mouse RPE/choroid
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 19 2019

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (liver, spleen)
Specification
liver, spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 11 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 10 2014

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