Anti-IL-34 antibody (ab74548)

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Overview

  • Product name

    Anti-IL-34 antibody
    See all IL-34 primary antibodies

  • Description

    Rabbit polyclonal to IL-34

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human IL-34 (N terminal). (ACD13474)

  • Positive control

    • Human brain tissue lysate

  • General notes

    Previously labelled as Interleukin 34.

Properties

Applications

Our Abpromise guarantee covers the use of ab74548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.

  • Tissue specificity

    Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

  • Sequence similarities

    Belongs to the IL-34 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C16orf77 antibody
    • Chromosome 16 open reading frame 77 antibody
    • FPT025 antibody
    • IL 34 antibody
    • IL-34 antibody
    • Il34 antibody
    • IL34_HUMAN antibody
    • Interleukin 34 antibody
    • Interleukin-34 antibody
    • Interleukin34 antibody
    • MGC34647 antibody
    see all

Images

  • Western blot - Anti-IL-34 antibody (ab74548)
    Western blot - Anti-IL-34 antibody (ab74548)
    Anti-IL-34 antibody (ab74548) at 1 µg/ml + Recombinant human IL-34 protein at 0.001 µg

    Predicted band size: 27 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-IL-34 antibody (ab74548)
    Western blot - Anti-IL-34 antibody (ab74548)
    All lanes : Anti-IL-34 antibody (ab74548) at 0.25 µg/ml

    Lane 1 : Human brain tissue lysate, minus immunising peptide
    Lane 2 : Human brain tissue lysate, plus immmunising peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 27 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Walker DG  et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). Read more (PubMed: 28848420) »
See 1 Publication for this product

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