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    il-34-antibody-ab74548.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-IL-34 antibody (ab74548)

  • Datasheet
  • SDS
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Western blot - Anti-IL-34 antibody (ab74548)
  • Western blot - Anti-IL-34 antibody (ab74548)

Key features and details

  • Rabbit polyclonal to IL-34
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human IL-34 protein (His tag) (ab234967)

View more associated products

Overview

  • Product name

    Anti-IL-34 antibody
    See all IL-34 primary antibodies
  • Description

    Rabbit polyclonal to IL-34
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human IL-34 (N terminal). (ACD13474)

  • Positive control

    • Human brain tissue lysate
  • General notes

    Previously labelled as Interleukin 34.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IL-34 protein (His tag) (ab234967)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa).
Notes
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.
  • Tissue specificity

    Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
  • Sequence similarities

    Belongs to the IL-34 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q6ZMJ4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 146433 Human
    • Omim: 612081 Human
    • SwissProt: Q6ZMJ4 Human
    • Unigene: 461214 Human
    • Alternative names

      • C16orf77 antibody
      • Chromosome 16 open reading frame 77 antibody
      • FPT025 antibody
      • IL 34 antibody
      • IL-34 antibody
      • Il34 antibody
      • IL34_HUMAN antibody
      • Interleukin 34 antibody
      • Interleukin-34 antibody
      • Interleukin34 antibody
      • MGC34647 antibody
      see all

    Images

    • Western blot - Anti-IL-34 antibody (ab74548)
      Western blot - Anti-IL-34 antibody (ab74548)
      Anti-IL-34 antibody (ab74548) at 1 µg/ml + Recombinant human IL-34 protein at 0.001 µg

      Predicted band size: 27 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-IL-34 antibody (ab74548)
      Western blot - Anti-IL-34 antibody (ab74548)
      All lanes : Anti-IL-34 antibody (ab74548) at 0.25 µg/ml

      Lane 1 : Human brain tissue lysate, minus immunising peptide
      Lane 2 : Human brain tissue lysate, plus immmunising peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 27 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab74548? Please let us know so that we can cite the reference in this datasheet.

    ab74548 has been referenced in 1 publication.

    • Walker DG  et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). PubMed: 28848420

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