Anti-IL-34 antibody (ab74548)
Key features and details
- Rabbit polyclonal to IL-34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IL-34 antibody
See all IL-34 primary antibodies -
Description
Rabbit polyclonal to IL-34 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human IL-34 (N terminal). (ACD13474)
-
Positive control
- Human brain tissue lysate
-
General notes
Previously labelled as Interleukin 34.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa).
|
| Notes |
|---|
|
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa). |
Target
-
Function
Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R. -
Tissue specificity
Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. -
Sequence similarities
Belongs to the IL-34 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 146433 Human
- Omim: 612081 Human
- SwissProt: Q6ZMJ4 Human
- Unigene: 461214 Human
-
Alternative names
- C16orf77 antibody
- Chromosome 16 open reading frame 77 antibody
- FPT025 antibody
see all
Images
-
Western blot - Anti-IL-34 antibody (ab74548)Anti-IL-34 antibody (ab74548) at 1 µg/ml + Recombinant human IL-34 protein at 0.001 µg
Predicted band size: 27 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Western blot - Anti-IL-34 antibody (ab74548)All lanes : Anti-IL-34 antibody (ab74548) at 0.25 µg/ml
Lane 1 : Human brain tissue lysate, minus immunising peptide
Lane 2 : Human brain tissue lysate, plus immmunising peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 27 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab74548 has been referenced in 1 publication.
- Walker DG et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). PubMed: 28848420